Kinard Lucas A, Dahlin Rebecca L, Henslee Allan M, Spicer Patrick P, Chu Chia-Ye, Tabata Yasuhiko, van den Beucken Jeroen J J P, Jansen John A, Young Simon, Wong Mark E, Kasper F Kurtis, Mikos Antonios G
Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas.
J Biomed Mater Res A. 2014 Jul;102(7):2079-88. doi: 10.1002/jbm.a.34878. Epub 2013 Jul 30.
The objective of the present study was to develop a preclinical animal model for evaluating bone augmentation and to examine the level of bone augmentation induced by hydrogel composites. Design criteria outlined for the development of the animal model included rigid immobilization of bilateral implants apposed to the parietal bone of the rat, while avoiding the calvarial sutures. The animal model was evaluated through the implantation of hydrogel composites of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles releasing bone morphogenetic protein-2 (BMP-2). The BMP-2 release profile was varied and compared to the implantation of a material control without BMP-2. Each hydrogel composite was implanted within a polypropylene cassette, which was immobilized to the calvarial bone using screws, and empty cassettes were implanted as a control. The design criteria for the animal model were realized; however, the level of bone augmentation did not vary between any of the groups after 4 weeks. Osteoclastic bone resorption occurred to a higher extent in groups releasing BMP-2, but the cause could not be elucidated. In conclusion, a promising bone augmentation model was established in the rat; however, refinement of the hydrogel composites was suggested to optimize the constructs for bone augmentation applications.
本研究的目的是开发一种用于评估骨增量的临床前动物模型，并检测水凝胶复合材料诱导的骨增量水平。为开发该动物模型概述的设计标准包括将双侧植入物牢固固定于大鼠顶骨，同时避开颅缝。通过植入聚（乙二醇富马酸酯）（OPF）和释放骨形态发生蛋白-2（BMP-2）的明胶微粒的水凝胶复合材料对该动物模型进行评估。改变BMP-2的释放曲线，并与植入不含BMP-2的材料对照进行比较。将每种水凝胶复合材料植入聚丙烯盒内，用螺钉将其固定于颅骨，植入空盒作为对照。实现了动物模型的设计标准；然而，4周后，任何一组之间的骨增量水平均无差异。在释放BMP-2的组中，破骨细胞性骨吸收程度更高，但原因尚不清楚。总之，在大鼠中建立了一个有前景的骨增量模型；然而，建议对水凝胶复合材料进行改进，以优化用于骨增量应用的构建体。
