Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas, USA.
Pharm Res. 2013 Sep;30(9):2332-43. doi: 10.1007/s11095-013-1077-5. Epub 2013 May 18.
Hydrogel composites of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles (GMs) were investigated as carriers of bone morphogenetic protein-2 (BMP-2) for bone tissue engineering applications.
Hydrogel composites with different physical characteristics were prepared by changing the amount and type (acidic vs. basic) of gelatin incorporated in the OPF bulk phase. Composites with differing physical properties (degradation, swelling, and mechanical properties) and differing BMP-2 loading phase were investigated to determine the effect of these factors on BMP-2 release profiles over 28 days.
Overall, higher gelatin amount increased the degradation and swelling of composites, and acidic GMs further increased the degradation and swelling and reduced the compressive modulus of the composites. The most significant factor affecting the release of BMP-2 from composites was the loading phase of the growth factor: GM loading reduced the burst release, increased BMP-2 release during the later phases of the experiment, and increased the cumulative release in faster degrading samples.
The results indicate that the physical properties and the BMP-2 release kinetics of hydrogel composites can be controlled by adjusting multiple parameters at the time of the hydrogel composite fabrication.
研究聚（乙二醇琥珀酸酯）（OPF）与明胶微球（GMs）的水凝胶复合材料作为骨形态发生蛋白-2（BMP-2）载体在骨组织工程中的应用。
通过改变 OPF 本体相中掺入的明胶的量和类型（酸性与碱性），制备具有不同物理特性的水凝胶复合材料。研究了具有不同物理特性（降解、溶胀和机械性能）和不同 BMP-2 加载阶段的复合材料，以确定这些因素对 28 天内 BMP-2 释放曲线的影响。
总的来说，明胶含量的增加会增加复合材料的降解和溶胀，而酸性 GMs 会进一步增加降解和溶胀，并降低复合材料的压缩模量。影响 BMP-2 从复合材料中释放的最重要因素是生长因子的加载阶段：GM 加载减少了突释，增加了实验后期 BMP-2 的释放，并增加了在降解较快的样品中的累积释放。
结果表明，通过在水凝胶复合材料制备时调整多个参数，可以控制水凝胶复合材料的物理性质和 BMP-2 释放动力学。
