Ball Jordan P, Mound Brittnee A, Nino Juan C, Allen Josephine B
Department of Materials Science & Engineering, University of Florida, 100 Rhines Hall, Gainesville, Florida, 32611.
J Biomed Mater Res A. 2014 Jul;102(7):2089-95. doi: 10.1002/jbm.a.34879. Epub 2013 Jul 30.
The potential of barium titanate (BT) to be electrically active makes it a material of interest in regenerative medicine. To enhance the understanding of this material for orthopedic applications, the in vitro biocompatibility of porous BT fabricated using a direct foaming technique was investigated. Characterization of the resultant foams yielded an overall porosity between 50 and 70% with average pore size in excess of 30 µm in diameter. A mouse osteoblast (7F2) cell line was cultured with the BT to determine the extent of the foams' toxicity using a LDH assay. After 72 h, BT foams showed a comparable cytotoxicity of 6.4 ± 0.8% to the 8.4 ± 1.5% of porous 45S5 Bioglass®. The in vitro inflammatory response elicited from porous BT was measured as a function of tumor necrosis factor alpha (TNF-α) secreted from a human monocytic leukemia cell line (THP-1). Results indicate that the BT foams do not cause a significant inflammatory response, eliciting a 9.4 ± 1.3 pg of TNF-α per mL of media compared with 20.2 ± 2.3 pg/mL from untreated cells. These results indicate that porous BT does not exhibit short term cytotoxicity and has potential for orthopedic tissue engineering applications.
钛酸钡（BT）具有电活性的特性使其成为再生医学中备受关注的材料。为了更深入了解这种材料在骨科应用中的情况，研究了采用直接发泡技术制备的多孔BT的体外生物相容性。对所得泡沫材料的表征结果显示，其总体孔隙率在50%至70%之间，平均孔径超过30 µm。使用小鼠成骨细胞（7F2）细胞系与BT共同培养，通过乳酸脱氢酶（LDH）测定法来确定泡沫材料的毒性程度。72小时后，BT泡沫材料的细胞毒性为6.4±0.8%，与多孔45S5生物活性玻璃®的8.4±1.5%相当。通过测量人单核细胞白血病细胞系（THP-1）分泌的肿瘤坏死因子α（TNF-α）来评估多孔BT引发的体外炎症反应。结果表明，BT泡沫材料不会引起显著的炎症反应，每毫升培养基中引发的TNF-α为9.4±1.3 pg，而未处理细胞分泌的TNF-α为20.2±2.3 pg/mL。这些结果表明，多孔BT不具有短期细胞毒性，在骨科组织工程应用中具有潜力。
