Ball Jordan P, Mound Brittnee A, Monsalve Adam G, Nino Juan C, Allen Josephine B
Department of Materials Science and Engineering, University of Florida, Gainesville, Florida.
J Biomed Mater Res A. 2015 Jan;103(1):8-15. doi: 10.1002/jbm.a.35137. Epub 2014 Mar 5.
Ceria ceramics have the unique ability to protect cells from free radical-induced damage, making them materials of interest for biomedical applications. To expand upon the understanding of the potential of ceria as a biomaterial, porous ceria, fabricated via direct foaming, was investigated to assess its biocompatibility and its ability to scavenge free radicals. A mouse osteoblast (7F2) cell line was cultured with the ceria foams to determine the extent of the foams' toxicity. Toxicity assessments indicate that mouse osteoblasts cultured directly on the ceria scaffold for 72 h did not show a significant (p > 0.05) increase in toxicity, but rather show comparable toxicity to cells cultured on porous 45S5 Bioglass. The in vitro inflammatory response elicited from porous ceria foams was measured as a function of tumor necrosis factor alpha (TNF-α) secreted from a human monocytic leukemia cell line. Results indicate that the ceria foams do not cause a significant inflammatory response, eliciting a response of 27.1 ± 7.1 pg mL(-1) of TNF-α compared to 36.3 ± 5.8 pg mL(-1) from cells on Bioglass, and 20.1 ± 2.9 pg mL(-1) from untreated cells. Finally, we report cellular toxicity in response to free radicals from tert-butyl hydroperoxide with and without foamed ceria. Our preliminary results show that the foamed ceria is able to decrease the toxic effect of induced oxidative stress. Collectively, this study demonstrates that foamed ceria scaffolds do not activate an inflammatory response, and show potential free radical scavenging ability, thus they have promise as an orthopedic biomaterial.
二氧化铈陶瓷具有独特的能力，能够保护细胞免受自由基诱导的损伤，这使其成为生物医学应用中备受关注的材料。为了更深入地了解二氧化铈作为生物材料的潜力，对通过直接发泡制备的多孔二氧化铈进行了研究，以评估其生物相容性和清除自由基的能力。将小鼠成骨细胞（7F2）细胞系与二氧化铈泡沫一起培养，以确定泡沫的毒性程度。毒性评估表明，直接在二氧化铈支架上培养72小时的小鼠成骨细胞毒性没有显著（p>0.05）增加，而是与在多孔45S5生物玻璃上培养的细胞具有相当的毒性。测量了多孔二氧化铈泡沫引发的体外炎症反应，该反应是人类单核细胞白血病细胞系分泌的肿瘤坏死因子α（TNF-α）的函数。结果表明，二氧化铈泡沫不会引起显著的炎症反应，引发的TNF-α反应为27.1±7.1 pg mL-1，而生物玻璃上的细胞为36.3±5.8 pg mL-1，未处理细胞为20.1±2.9 pg mL-1。最后，我们报告了有无泡沫二氧化铈时细胞对叔丁基过氧化氢自由基的毒性反应。我们的初步结果表明，泡沫二氧化铈能够降低诱导的氧化应激的毒性作用。总的来说，这项研究表明泡沫二氧化铈支架不会激活炎症反应，并显示出潜在的自由基清除能力，因此它们有望成为一种骨科生物材料。
