Omer Hélène, McDowell Andrew, Alexeyev Oleg A
Department of Medical Biosciences/Pathology, Umeå University, Umeå, Sweden.
Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, C-TRIC Building, Altnagelvin Area Hospital, University of Ulster, Londonderry, UK.
Clin Dermatol. 2017 Mar-Apr;35(2):118-129. doi: 10.1016/j.clindermatol.2016.10.003. Epub 2016 Oct 27.
Acne vulgaris is a chronic inflammatory skin condition classified by the Global Burden of Disease Study as the eighth most prevalent disease worldwide. The pathophysiology of the condition has been extensively studied, with an increase in sebum production, abnormal keratinization of the pilosebaceous follicle, and an inflammatory immune response all implicated in its etiology. One of the most disputed points, however, is the role of the gram-positive anaerobic bacterium Propionibacterium acnes in the development of acne, particularly when this organism is also found in normal sebaceous follicles of healthy skin. Against this background, we now describe the different sampling strategies that have been adopted for qualitative and quantitative study of P acnes within intact hair follicles of the skin and discuss the strengths and weaknesses of such methodologies for investigating the role of P acnes in the development of acne.
寻常痤疮是一种慢性炎症性皮肤病，根据全球疾病负担研究，它是全球第八大最普遍的疾病。该病症的病理生理学已得到广泛研究，皮脂分泌增加、毛囊皮脂腺异常角化以及炎症免疫反应均与其病因有关。然而，最具争议的问题之一是革兰氏阳性厌氧菌痤疮丙酸杆菌在痤疮发病中的作用，尤其是当这种微生物也存在于健康皮肤的正常皮脂腺毛囊中时。在此背景下，我们现在描述了为定性和定量研究皮肤完整毛囊内的痤疮丙酸杆菌而采用的不同采样策略，并讨论了这些方法在研究痤疮丙酸杆菌在痤疮发病中的作用方面的优缺点。
