Department of Biotechnology, University of Verona Verona, Italy.
Front Plant Sci. 2013 Jul 26;4:280. doi: 10.3389/fpls.2013.00280. eCollection 2013.
Hyperaccumulator/hypertolerant plant species have evolved strategies allowing them to grow in metal-contaminated soils, where they accumulate high concentrations of heavy metals in their shoots without signs of toxicity. The mechanisms that allow enhanced metal uptake, root-to-shoot translocation and detoxification in these species are not fully understood. Complementary approaches such as transcriptomic-based DNA microarrays and proteomics have recently been used to gain insight into the molecular pathways evolved by metal hyperaccumulator/hypertolerant species. Proteomics has the advantage of focusing on the translated portion of the genome and it allows to analyze complex networks of proteins. This review discusses the recent analysis of metal hyperaccumulator/hypertolerant plant species using proteomics. Changes in photosynthetic proteins, sulfur, and glutathione metabolism, transport, biotic and xenobiotic defenses as well as the differential regulation of proteins involved in signaling and secondary metabolism are discussed in relation to metal hyperaccumulation. We also consider the potential contribution of several proteins to the hyperaccumulation phenotype.
超积累/高耐受植物物种已经进化出了一些策略，使它们能够在重金属污染的土壤中生长，在这些土壤中，它们的地上部分会积累大量的重金属，而没有毒性的迹象。这些物种中增强金属吸收、根到梢转运和解毒的机制尚未完全了解。转录组学为基础的 DNA 微阵列和蛋白质组学等补充方法最近被用于深入了解金属超积累/高耐受物种进化的分子途径。蛋白质组学的优点是专注于基因组的翻译部分，它允许分析复杂的蛋白质网络。本综述讨论了最近利用蛋白质组学对金属超积累/高耐受植物物种的分析。与金属超积累有关的讨论包括光合作用蛋白、硫和谷胱甘肽代谢、运输、生物和异生物质防御以及参与信号转导和次生代谢的蛋白质的差异调控。我们还考虑了几种蛋白质对超积累表型的潜在贡献。
