BMC Complement Altern Med. 2013 Jul 30;13:198. doi: 10.1186/1472-6882-13-198.
Germinated brown rice (GBR) is gaining momentum in the area of biomedical research due to its increased use as a nutraceutical for the management of diseases. The effect of GBR on the reproductive organs of oophorectomised rats was studied using the gross, cytological, histological and immunohistochemical changes, with the aim of reducing atrophy and dryness of the genital organs in menopause.
Experimental rats were divided into eight groups of six rats per group. Groups 1, 2 and 3 (sham-operated (SH), oophorectomised without treatment (OVX) and oophorectomised treated with 0.2 mg/kg oestrogen, respectively) served as the controls. The groups 4,5,6,7 and 8 were treated with 20 mg/kg Remifemin, 200 mg/kg of GBR, ASG, oryzanol and GABA, respectively. All treatments were administered orally, once daily for 8 weeks. Vaginal smear cytology was done at the 7th week on all the rats. The weight and dimensions of the uterus and vagina were determined after sacrifice of the rats. Uterine and vaginal tissues were taken for histology and Immunohistochemical examinations.
GBR and its bioactives treated groups significantly increased the weight and length of both the uterus and the vagina when compared to Oophorectomised non-treated group (OVX-non-treated) (p < 0.05). Significant changes were observed in the ratio of cornified epithelial cells and number of leucocytes in the vaginal cytology between the oophorectomised non-treated and treated groups. There was also an increase in the luminal and glandular epithelial cells activity in the treated compared with the untreated groups histologically. Immunohistochemical staining showed specific proliferating cell nuclear antigen (PCNA) in the luminal and glandular epithelium of the treated groups, which was absent in the OVX-non-treated group. GBR improved the length and weight of the uterus and also increased the number of glandular and luminal cells epithelia of the vagina.
GBR and its bioactives could be a potential alternative in improving reproductive system atrophy, dryness and discomfort during menopause.
由于发芽糙米（GBR）作为一种用于管理疾病的营养保健品的使用增加，因此在生物医学研究领域备受关注。本研究旨在通过大体观察、细胞学、组织学和免疫组织化学变化来研究 GBR 对去卵巢大鼠生殖器官的影响，以减少绝经后生殖器器官的萎缩和干燥。
将实验大鼠分为 8 组，每组 6 只。第 1、2 和 3 组（假手术（SH）、未治疗去卵巢（OVX）和用 0.2mg/kg 雌激素治疗的去卵巢）作为对照组。第 4、5、6、7 和 8 组分别用 20mg/kg 瑞美芬、200mg/kg GBR、ASG、谷维素和 GABA 治疗。所有治疗均为口服，每日 1 次，共 8 周。所有大鼠在第 7 周时进行阴道涂片细胞学检查。处死大鼠后测量子宫和阴道的重量和尺寸。取子宫和阴道组织进行组织学和免疫组织化学检查。
与未治疗去卵巢组（OVX-未治疗）相比，GBR 和其生物活性物质处理组的子宫和阴道重量和长度显著增加（p<0.05）。在阴道细胞学中，去卵巢未治疗组和治疗组之间观察到角化上皮细胞和白细胞数量的比例发生了显著变化。与未治疗组相比，治疗组的腔上皮和腺上皮细胞活性也增加。免疫组织化学染色显示，在治疗组的腔上皮和腺上皮中存在特异性的增殖细胞核抗原（PCNA），而在 OVX-未治疗组中则不存在。GBR 改善了子宫的长度和重量，并增加了阴道的腺上皮和腔上皮细胞数量。
GBR 及其生物活性物质可能是改善绝经后生殖系统萎缩、干燥和不适的潜在替代方法。
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