Parveen Z, Nawaz S, Siddique S, Shahzad K
PCSIR Laboratories Complex, Ferozpur Road, Lahore 54600, Pakistan.
Indian J Pharm Sci. 2013 Jan;75(1):117-22. doi: 10.4103/0250-474X.113544.
The essential oil from the leaves of Curcuma longa L. Kasur variety grown in Pakistan was extracted by hydro-distillation. Chemical constituents of the essential oil were identified by gas chromatography/mass spectrometry. The chromatographic analysis of oil showed 25 constituents, out of which nine chemical constituents were identified. The eucalyptol (10.27%) was the major component of the essential oil. α-pinene (1.50%), β-phellandrene (2.49%), β-pinene (3.57%), limonene (2.73%), 1,3,8-p-menthatriene (1.76%), ascaridole epoxide (1.452%), 2-methylisoborneol (2.92%), 5-isopropyl-6-methyl-hepta-3, dien-2-ol (2.07%) were also present in considerable quantity. The antimicrobial properties of leaves of Curcuma longa were tested by disc diffusion method against various human pathogens, including eight fungal and five bacterial strains. Essential oil showed maximum resistance against Fusarium miniformes MAY 3629 followed by Bacillus subtilis ATCC 6633 whereas; it exhibited least resistance against Fusarium oxysporium ATCC 48122. The results of the antimicrobial assay revealed that essential oil showed significant inhibitory activity against the tested organisms.
采用水蒸馏法提取了在巴基斯坦种植的卡苏尔品种姜黄叶片中的精油。通过气相色谱/质谱联用仪鉴定了该精油的化学成分。精油的色谱分析显示有25种成分，其中9种化学成分得到了鉴定。桉叶油素（10.27%）是该精油的主要成分。α-蒎烯（1.50%）、β-水芹烯（2.49%）、β-蒎烯（3.57%）、柠檬烯（2.73%）、1,3,8-对薄荷三烯（1.76%）、环氧蛔蒿素（1.452%）、2-甲基异龙脑（2.92%）、5-异丙基-6-甲基-3,7-二烯-2-醇（2.07%）的含量也相当可观。采用纸片扩散法对姜黄叶片的抗菌性能进行了测试，针对包括8种真菌和5种细菌菌株在内的多种人类病原体。精油对极小镰刀菌MAY 3629的抗性最强，其次是枯草芽孢杆菌ATCC 6633；而对尖孢镰刀菌ATCC 48122的抗性最弱。抗菌试验结果表明，该精油对受试微生物具有显著的抑制活性。
