Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Central Research Laboratory, Deputy of Research, Kerman University of Medical Sciences, Kerman, Iran.
BMC Complement Altern Med. 2019 Jul 26;19(1):187. doi: 10.1186/s12906-019-2527-3.
One of the most important ways to cure hydatid cysts is surgery. Today, available chemical drugs have shown severe complications during hydatid cyst surgery. Here we investigated theefficacy and safety of Curcuma longa (C. longa) essential oil (CLEO) against hydatid cysts protoscoleces.
Here, the collected protoscoleces from fertile hydatid cysts were exposed to different concentrations of the CLEO (50-200 μl/mL) for 5-30 min in vitro and ex vivo. In addition, male NIH mice (48 mice) were applied to examine the safety of CLEO.
All protoscoleces were completely killed in 5th min of treatment to the doses of 200 μl /mL of CLEO. On the other hand, in the 10th min of treatment, CLEO entirely killed 100% of protoscoleces at the dose of 100 μL/mL. Other doses of CLEO, but, revealed a postponed activity. Although CLEO at the doses of 50, and 100 μL/mL exhibited no similar effect in the ex vivo analysis; but, at the dose of 200 μL/mL and an exposure time of 5 min, approximately 100% of protoscoleces were destroyed into the cyst. We found that after intra-peritoneal injection of the CLEO for 14 days, although blood and biochemical parameters were changed; but there was no significant difference in comparison with the control group (p > 0.05).
This research demonstrated that C. longa revealed the promising scolicidal effects against protoscoleces in vitro and in vivo, of course, after additional tests; it might be considered as an herbal scolicidal drug in order to decrease the threat of discharge of protoscoleces through hydatid cyst surgery. Nevertheless, supplementary studies will be desired to prove the current findings by examination the essential oil in a clinical setting.
治疗包虫囊肿最重要的方法之一是手术。如今，现有的化学药物在包虫囊肿手术中显示出严重的并发症。在这里，我们研究了姜黄精油（CLEO）对包虫囊肿原头蚴的疗效和安全性。
从富营养的包虫囊肿中收集原头节，在体外和离体条件下，将其暴露于不同浓度的 CLEO（50-200μl/ml）中 5-30 分钟。此外，还应用雄性 NIH 小鼠（48 只）来检验 CLEO 的安全性。
在 200μl/ml 的剂量下，所有原头节在治疗的第 5 分钟内完全被杀死。另一方面，在治疗的第 10 分钟，在 100μl/ml 的剂量下，CLEO 完全杀死了 100%的原头节。但是，其他剂量的 CLEO 显示出延迟的活性。虽然 CLEO 在 50 和 100μl/ml 的剂量下在离体分析中没有类似的效果；但是，在 200μl/ml 的剂量和 5 分钟的暴露时间下，大约 100%的原头节被破坏成囊肿。我们发现，在腹腔注射 CLEO 14 天后，虽然血液和生化参数发生了变化；但与对照组相比，没有显著差异（p>0.05）。
本研究表明，姜黄在体外和体内对原头蚴具有有希望的杀囊效果，当然，在进一步的测试后；它可以被认为是一种草药杀囊药物，以减少通过包虫囊肿手术排出原头节的威胁。然而，需要进行补充研究，以通过在临床环境中检查精油来证明当前的发现。
