Murugan T, Wins J Albino, Murugan M
Microbilogy Unit, Kamini Research Foundation, Thuckalay 629 175, India.
Indian J Pharm Sci. 2013 Jan;75(1):122-5. doi: 10.4103/0250-474X.113546.
Plants produce a wide variety of phytochemical constituents, which are secondary metabolites and are used either directly or indirectly in the pharmaceutical industry. 'For centuries, man has effectively used various components of plants or their extracts for the treatment of many diseases, including bacterial infections. In the present study methanol, chloroform and aqueous extracts of Cassia auriculata leaf were subjected for antimicrobial activity by well-diffusion method against six bacterial strains namely Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis. The results revealed that the methanol and chloroform extracts exhibited strong inhibitory activity against all the tested organisms (zone of inhibition of 12-20 mm), except Pseudomonas aeruginosa (zone of inhibition 10 mm or nil). The aqueous extracts showed moderate activity by 'Zone of inhibition ≤12 or nil). The extracts were screened for their phytochemical constituents by standard protocols' and were shown to contain carbohydrates, proteins, alkaloids, flavonoids, steroids, saponins and tannins. The antibacterial activity of these extracts is possibly linked to the presence of flavonoids, steroid, saponins and/or tannins. Further studies are needed to determine the precise active principles from Cassia auriculata.
植物产生种类繁多的植物化学成分，这些成分属于次生代谢产物，在制药工业中直接或间接地得到应用。几个世纪以来，人类有效地利用植物的各种成分或其提取物来治疗包括细菌感染在内的多种疾病。在本研究中，采用打孔扩散法对耳叶决明叶的甲醇提取物、氯仿提取物和水提取物针对蜡样芽孢杆菌、金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和奇异变形杆菌这六种细菌菌株进行了抗菌活性测试。结果显示，甲醇提取物和氯仿提取物对所有受试微生物均表现出较强的抑制活性（抑菌圈直径为12 - 20毫米），但对铜绿假单胞菌除外（抑菌圈直径为10毫米或无抑菌圈）。水提取物表现出中等活性（抑菌圈直径≤12毫米或无抑菌圈）。通过标准方法对提取物的植物化学成分进行了筛选，结果表明其含有碳水化合物、蛋白质、生物碱、黄酮类化合物、甾体、皂苷和单宁。这些提取物的抗菌活性可能与黄酮类化合物、甾体、皂苷和/或单宁的存在有关。需要进一步研究以确定耳叶决明中确切的活性成分。
