Kausar Farzana, Farooqi Muhammad-Awais, Farooqi Hafiz-Muhammad-Umer, Salih Abdul-Rahim-Chethikkattuveli, Khalil Atif-Ali-Khan, Kang Chul-Woong, Mahmoud Mohamed H, Batiha Gaber-El-Saber, Choi Kyung-Hyun, Mumtaz Abdul-Samad
Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Mechatronics Engineering, Jeju National University, Jeju-si 63243, Korea.
Life (Basel). 2021 Jul 5;11(7):656. doi: 10.3390/life11070656.
The appearance of novel microbial resistance, diverse cancer ailment and several other morbidities such as appetite loss, hair loss, anemia, cell damage, etc., are among most critical situation that keeps the phytochemical quest on. Thus, this study characterized the antimicrobial, antioxidant, and anticancer potentials of a rarely accessed gled (AC) population thriving in a remote Palas Valley in northern Pakistan. Leaf extracts of the plant were prepared in organic solvents with different polarities through maceration. Extracts were subjected to antimicrobial, antioxidant, and anticancer activities using agar well, DPPH and cell viability assays. methanolic extract (ACM) significantly inhibited bacterial growth, followed by -butanolic extract (ACB) with the second-highest bacterial inhibition. Similar activity was observed against mycelial growth inhibition in plant-fungal pathogen by ACM and ACB. However, human pathogenic fungi did not affect much by extracts. In antioxidant assessment, the chloroform extract (ACC) showed strong scavenging activity and in cytotoxic evaluation, extracts restricted growth proliferation in cancer cells. The inhibitory evidence of extracts, potent scavenging ability, and low cell viability of human-derived cell lines supports the antimicrobial, antioxidant and anticancerous potential of It advances our quest for natural product research.
新型微生物耐药性的出现、多种癌症疾病以及其他几种病症，如食欲不振、脱发、贫血、细胞损伤等，是促使人们不断探索植物化学物质的最关键情况之一。因此，本研究对生长在巴基斯坦北部偏远的帕拉斯山谷中一种鲜为人知的皂荚种群的抗菌、抗氧化和抗癌潜力进行了表征。通过浸渍法在具有不同极性的有机溶剂中制备该植物的叶提取物。使用琼脂孔、DPPH和细胞活力测定法对提取物进行抗菌、抗氧化和抗癌活性测试。甲醇提取物（ACM）显著抑制细菌生长，其次是丁醇提取物（ACB），其细菌抑制作用次之。ACM和ACB对植物真菌病原体的菌丝体生长抑制也有类似活性。然而，提取物对人类致病真菌的影响不大。在抗氧化评估中，氯仿提取物（ACC）表现出较强的清除活性，在细胞毒性评估中，提取物抑制癌细胞的生长增殖。提取物的抑制证据、强大的清除能力以及人源细胞系的低细胞活力支持了其抗菌、抗氧化和抗癌潜力。这推动了我们对天然产物研究的探索。
