海南长吻鮈（Hemibarbus medius Yue）（硬骨鱼纲：鲤科）的线粒体全基因组
The complete mitochondrial genome of Hainanese barbel steed Hemibarbus medius Yue (Teleostei: Cyprinidae).
作者信息
Chen I-Shiung, Liao Chiao-Ru, Shao Yi-Ta
机构信息
a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan and.
b Institute of Cellular and Organismic Biology, Academia Sinica , Taipei , Taiwan.
出版信息
Mitochondrial DNA. 2015;26(5):736-8. doi: 10.3109/19401736.2013.845765. Epub 2014 Jan 27.
In this study, the complete mitogenome sequence of cyprinid fish, Hainanese barbel steed, Hemibarbus medius Yue (Teleostei: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,614 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region (CR). CR of 933 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of Hemibarbus medius is 29.8% for A, 27.2% for C, 25.9% for T and 17.1% for G, with a slight AT bias of 55.7%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.
在本研究中，采用长聚合酶链反应方法扩增并测序了鲤科鱼类海南长须鳅鮀（Hemibarbus medius Yue，硬骨鱼纲：鲤科）的完整线粒体基因组序列。该线粒体基因组由16,614个碱基对（bp）组成，具有典型的脊椎动物线粒体基因排列，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区（CR）。长度为933 bp的CR位于tRNA(Pro)和tRNA(Phe)之间。海南长须鳅鮀的总体碱基组成为：A占29.8%，C占27.2%，T占25.9%，G占17.1%，AT含量略偏多，为55.7%。完整的线粒体基因组不仅可为鲤科鱼类的同属物种，也可为更高分类阶元的进一步系统发育分析提供相当重要的DNA分子数据。
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