Nizeyimana Gerald, Mwiine Frank N, Ayebazibwe Chrisostom
College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Uganda.
J S Afr Vet Assoc. 2013;84(1):E1-5. doi: 10.4102/jsava.v84i1.943.
A study was conducted in the Luwero and Nakasongola districts in central Uganda to determine and compare the prevalence and distribution of antibodies against Brucella abortus in cattle under contrasting husbandry practices, using two serological tests. Three hundred and fifteen serum samples were systematically sampled from 29 farms and subsequently tested using the Rose Bengal plate test (RBPT) and Indirect Antibody Enzyme Linked Immunosorbent Assay (I-ELISA). The overall prevalence of antibodies against Brucella abortus in the Nakasongola and Luwero districts was 2.4% and 4.7% on RBPT, compared with 1.2% and 3.34 % on I-ELISA. There was no significant difference between the results obtained by RBPT and indirect antibody ELISA (p > 0.05). It was noted that antibodies against Brucella abortus were widely spread over different farms regardless of the cattle grazing system (p > 0.05). Based on the findings, it is feasible to use RBPT as a cheaper screening alternative for brucellosis. A comprehensive national brucellosis study should be undertaken to study the epidemiology and prevalence of brucellosis in Uganda.
在乌干达中部的卢韦罗区和纳卡松戈拉区开展了一项研究，旨在通过两种血清学检测方法，确定并比较不同饲养方式下牛群中抗布鲁氏菌流产菌抗体的流行率和分布情况。从29个农场系统采集了315份血清样本，随后使用玫瑰红平板试验（RBPT）和间接抗体酶联免疫吸附测定（I-ELISA）进行检测。在纳卡松戈拉区和卢韦罗区，RBPT检测出的抗布鲁氏菌流产菌抗体总体流行率分别为2.4%和4.7%，而I-ELISA检测结果分别为1.2%和3.34%。RBPT和间接抗体ELISA获得的结果之间无显著差异（p>0.05）。值得注意的是，无论牛的放牧系统如何，抗布鲁氏菌流产菌抗体在不同农场中广泛分布（p>0.05）。基于这些发现，使用RBPT作为布鲁氏菌病更廉价的筛查方法是可行的。应开展一项全面的全国性布鲁氏菌病研究，以研究乌干达布鲁氏菌病的流行病学和流行率。
