Seroprevalence, associated risk factors, and molecular detection of bovine brucellosis in rural areas of Egypt.

The study was carried out on six villages in northern Egypt to evaluate the epidemiological situation of bovine brucellosis among 989 unvaccinated household cattle by Rose Bengal Plate Test (RBPT) and indirect ELISA (iELISA) and to investigate the existence of Brucella DNA using real-time PCR in 100 milk and 100 sera from seropositive cattle and 50 sera from seronegative cattle. The overall seroprevalence was 20.7% and 23.7% by RBPT and iELISA, respectively. Based on the iELISA results, the seroprevalence was significantly (P < 0.05) higher in the village II (34.7%) and cattle > 7 years (30.1%). More males than females were non-significant seropositive (P = 0.6). There was 95% agreement between RBPT and iELISA, although iELISA showed a higher positivity rate (23.7%, 95% CI: 0.21-0.26) than RBPT (20.7%, 95% CI: 0.18-0.24). DNA of Brucella was confirmed in 16 milk samples by IS711 qPCR from seropositive cattle, however, no Brucella DNA was detected in serum samples tested positive and negative. Brucella abortus was the only species detected based on the alkB gene. Prevalence is highly related to the sampling site and the age of the animals. In conclusion, although qPCR is more accurate and commonly used in the diagnosis of most infectious diseases but in this situation iELISA is preferred and recommended for continuous screening and animal movement restriction and vaccination protocols, especially in high-risk areas.