Institut Européen de la Chimie et Biologie, CBMN-UMR5248, Université de Bordeaux 1, Pessac, France.
Nano Lett. 2013 Aug 14;13(8):3923-9. doi: 10.1021/nl4020149. Epub 2013 Aug 1.
We present a method of preparing and characterizing nanostructured bioactive motifs using a combination of nanoimprint lithography and surface functionalization. Nanodots were fabricated on silicon surfaces and modified with a cell-adhesive RGD peptide for studies in human mesenchymal stem cell adhesion and differentiation. We report that bioactive nanostructures induce mature focal adhesions on human mesenchymal stem cells with an impact on their behavior and dynamics specifically in terms of cell spreading, cell-material contact, and cell differentiation.
我们提出了一种使用纳米压印光刻和表面功能化相结合的方法来制备和表征纳米结构生物活性基序。在硅表面上制造纳米点，并通过细胞黏附 RGD 肽进行修饰，用于研究人骨髓间充质干细胞的黏附和分化。我们报告称，生物活性纳米结构在人骨髓间充质干细胞上诱导成熟的焦点黏附，并对其行为和动力学产生影响，特别是在细胞铺展、细胞-材料接触和细胞分化方面。
