Ren Fang, Yesildag Cigdem, Zhang Zhenfang, Lensen Marga C
Nanopatterned Biomaterials, Technische Universität Berlin, Sekr. TC 1, Strasse des 17. Juni 124, Berlin 10623, Germany.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Polymers (Basel). 2017 Apr 26;9(5):154. doi: 10.3390/polym9050154.
We report on a versatile and easy approach to micro-pattern gold nanoparticles (Au NPs) on 8-arm poly(ethylene glycol)-vinyl sulfone thiol (8PEG-VS-SH) hydrogels, and the application of these patterned Au NPs stripes in controlling cell adhesion. Firstly, the Au NPs were patterned on silicon wafers, and then they were transferred onto reactive, multifunctional 8PEG-VS-SH hydrogels. The patterned, micrometer-sized Au NPs stripes with variable spacings ranging from 20 μm to 50 μm were created by our recently developed micro-contact deprinting method. For this micro-contact deprinting approach, four different PEG-based stamp materials have been tested and it was found that the triblock copolymer PEG-PPG-PEG-(3BC) stamp established the best transfer efficiency and has been used in the ongoing work. After the successful creation of micro-patterns of Au NPs stripes on silicon, the patterns can be transferred conveniently and accurately to 8PEG-VS-SH hydrogel films. Subsequently these Au NPs patterns on 8PEG-VS-SH hydrogels have been investigated in cell culture with murine fibroblasts (L-929). The cells have been observed to adhere to and spread on those nano-patterned micro-lines in a remarkably selective and ordered manner.
我们报道了一种在八臂聚乙二醇 - 乙烯砜硫醇（8PEG - VS - SH）水凝胶上制备微图案化金纳米颗粒（Au NPs）的通用且简便的方法，以及这些图案化的Au NPs条纹在控制细胞黏附中的应用。首先，在硅片上制备Au NPs图案，然后将其转移到具有反应性的多功能8PEG - VS - SH水凝胶上。通过我们最近开发的微接触脱印法，制备出了间距在20μm至50μm之间变化的微米级图案化Au NPs条纹。对于这种微接触脱印方法，我们测试了四种不同的基于聚乙二醇的印章材料，发现三嵌段共聚物聚乙二醇 - 聚丙二醇 - 聚乙二醇（3BC）印章具有最佳的转移效率，并已用于后续工作。在硅片上成功制备出Au NPs条纹的微图案后，这些图案可以方便且准确地转移到8PEG - VS - SH水凝胶薄膜上。随后，在小鼠成纤维细胞（L - 929）的细胞培养中对8PEG - VS - SH水凝胶上的这些Au NPs图案进行了研究。观察到细胞以显著的选择性和有序方式黏附并铺展在那些纳米图案化的微线上。
