Liu Guo-Hong, Yao Ling, Gao Peng, Zhang Shu-Qun, Li Bing, Rensing Christopher, Li Wen-Qing, Zhou Shun-Gui
Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350003, People's Republic of China.
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People's Republic of China.
Curr Microbiol. 2025 Jul 24;82(9):406. doi: 10.1007/s00284-025-04376-x.
A Gram-stain-negative, rod-shaped, strictly aerobic bacterium strain SG148, within the family Rhizobiaceae, was isolated from rice leaves collected in Fujian Province, China. Phylogenetic analysis utilizing 16S rRNA gene sequences revealed that strain SG148 displayed a high sequence similarity of 95.2-95.8% to the strains of closely related species of Shinella and Rhizobium. The highest digital DNA-DNA hybridization (dDDH), core-proteome average amino acid identity (cpAAI), and average nucleotide identity (ANI) values between strain SG148 and related strains were estimated as 20.7%, 79.3%, and 73.7%, respectively. The predominant respiratory quinones in strain SG148 were identified as Q-9 and Q-10, while major polar lipids included diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine. Additionally, strain SG148 was capable of phosphate-solubilizing and siderophore-producing carrier, significantly promoting rice seedling growth. The DNA G + C content was determined to be 56.1%. Given its distinct phylogenetic and phenotypic characteristics, strain SG148 is considered to represent a novel genus, for which the name Oryzifoliimicrobium ureilyticus gen. nov., sp. nov. is proposed. The type strain is SG148 (= MCCC 1K08901 = KCTC 8337).
从中国福建省采集的水稻叶片中分离出一株革兰氏染色阴性、杆状、严格需氧的细菌菌株SG148，属于根瘤菌科。利用16S rRNA基因序列进行的系统发育分析表明，菌株SG148与希内拉属和根瘤菌属密切相关物种的菌株显示出95.2 - 95.8%的高序列相似性。菌株SG148与相关菌株之间的最高数字DNA - DNA杂交（dDDH）、核心蛋白质组平均氨基酸同一性（cpAAI）和平均核苷酸同一性（ANI）值分别估计为20.7%、79.3%和73.7%。菌株SG148中的主要呼吸醌被鉴定为Q - 9和Q - 10，而主要极性脂类包括双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。此外，菌株SG148能够溶解磷酸盐并产生铁载体，显著促进水稻幼苗生长。DNA G + C含量测定为56.1%。鉴于其独特的系统发育和表型特征，菌株SG148被认为代表一个新属，为此提出新属名解脲稻叶微菌属（Oryzifoliimicrobium），新种名解脲稻叶微菌（Oryzifoliimicrobium ureilyticus）。模式菌株为SG148（= MCCC 1K08901 = KCTC 8337）。
