Oryzifoliimicrobium ureilyticus gen. nov., sp. nov., a Novel Phosphate-Solubilizing and Siderophore-Producing Bacterium Within the Family Rhizobiaceae.

A Gram-stain-negative, rod-shaped, strictly aerobic bacterium strain SG148, within the family Rhizobiaceae, was isolated from rice leaves collected in Fujian Province, China. Phylogenetic analysis utilizing 16S rRNA gene sequences revealed that strain SG148 displayed a high sequence similarity of 95.2-95.8% to the strains of closely related species of Shinella and Rhizobium. The highest digital DNA-DNA hybridization (dDDH), core-proteome average amino acid identity (cpAAI), and average nucleotide identity (ANI) values between strain SG148 and related strains were estimated as 20.7%, 79.3%, and 73.7%, respectively. The predominant respiratory quinones in strain SG148 were identified as Q-9 and Q-10, while major polar lipids included diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine. Additionally, strain SG148 was capable of phosphate-solubilizing and siderophore-producing carrier, significantly promoting rice seedling growth. The DNA G + C content was determined to be 56.1%. Given its distinct phylogenetic and phenotypic characteristics, strain SG148 is considered to represent a novel genus, for which the name Oryzifoliimicrobium ureilyticus gen. nov., sp. nov. is proposed. The type strain is SG148 (= MCCC 1K08901 = KCTC 8337).