Nogi Yuichi, Mori Kozue, Uchida Hiromi, Hatada Yuji
Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Yokohama City University International Graduate School of Arts and Sciences, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
Int J Syst Evol Microbiol. 2015 Sep;65(9):2786-2790. doi: 10.1099/ijs.0.000333. Epub 2015 May 14.
A novel marine bacterial strain designated JAMH 011(T) was isolated from the cold-seep sediment in Sagami Bay, Japan. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, aerobic chemo-organotrophs and motile by means of a single polar flagellum. Growth occurred at temperatures below 31 °C, with the optimum at 25 °C. The major respiratory quinone was Q-10. The predominant fatty acid was C18 : 1ω7c. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated with members of the genus Shimia in the class Alphaproteobacteria, and the 16S rRNA gene sequence similarity of the novel isolate with the type strain of the closest related species, Shimia haliotis WM35(T), was 98.1%. The DNA G+C content of the novel strain was 57.3 mol%. The hybridization values for DNA-DNA relatedness between strain JAMH 011(T) and reference strains belonging to the genus Shimia were less than 9.4 ± 0.7%. Based on differences in taxonomic characteristics, the isolated strain represents a novel species of the genus Shimia, for which the name Shimia sagamensis sp. nov. is proposed. The type strain is JAMH 011(T) ( = JCM 30583(T) = DSM 29734(T)).
从日本相模湾冷泉沉积物中分离出一株新型海洋细菌菌株，命名为JAMH 011(T)。细胞革兰氏染色阴性，杆状，不产芽孢，好氧化学有机营养型，通过单根极生鞭毛运动。在31℃以下温度生长，最适温度为25℃。主要呼吸醌为Q-10。主要脂肪酸为C18 : 1ω7c。基于16S rRNA基因序列分析，分离菌株与α-变形菌纲Shimia属的成员密切相关，该新分离株与最密切相关物种Shimia haliotis WM35(T)的模式菌株的16S rRNA基因序列相似性为98.1%。新菌株的DNA G+C含量为57.3 mol%。菌株JAMH 011(T)与Shimia属参考菌株之间的DNA-DNA相关性杂交值小于9.4±0.7%。基于分类学特征的差异，分离菌株代表Shimia属的一个新物种，为此提出名称Shimia sagamensis sp. nov.。模式菌株为JAMH 011(T)（=JCM 30583(T)=DSM 29734(T)）。
