Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4540-4545. doi: 10.1099/ijs.0.050062-0. Epub 2013 Aug 1.
A novel Gram-stain-positive, facultatively anaerobic, non-motile and lactic-acid-producing bacterium, designated strain ORL-24(T), was isolated from the gut of the camel cricket, Diestrammena coreana. Optimal growth occurred at 37 °C, pH 8 and with 0 % (w/v) NaCl. The ratio of l-lactate to d-lactate in strain ORL-24(T) was 96 : 4. Lancefield antigen D was not detected. The strain was negative for oxidase activity and catalase activity. According to a phylogenetic analysis based on 16S rRNA gene sequences, strain ORL-24(T) was most closely related to the type strain of Enterococcus asini (96.9 % similarity). Comparative pheS and rpoA sequence analyses of strain ORL-24(T) indicated that the strain belonged to the genus Enterococcus. The major fatty acids were C16 : 0 and C18 : 1ω9c. The DNA G+C content was 41.3 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain ORL-24(T) represents a novel species of the genus Enterococcus, for which the name Enterococcus diestrammenae is proposed. The type strain is ORL-24(T) ( = KACC 16708(T) = JCM 18359(T)).
一株革兰氏阳性、兼性厌氧、非运动和产乳酸的细菌，被命名为 ORL-24(T) 菌株，从骆驼蟋蟀（Diestrammena coreana）的肠道中分离得到。最佳生长条件为 37°C、pH8 和 0%（w/v）NaCl。ORL-24(T) 菌株中 l-乳酸与 d-乳酸的比例为 96:4。未检测到 Lancefield 抗原 D。该菌株氧化酶和过氧化氢酶活性均为阴性。根据基于 16S rRNA 基因序列的系统发育分析，ORL-24(T) 菌株与马肠球菌（Enterococcus asini）的模式株最为密切相关（相似度为 96.9%）。ORL-24(T) 菌株的 pheS 和 rpoA 序列比较分析表明，该菌株属于肠球菌属。主要脂肪酸为 C16:0 和 C18:1ω9c。DNA G+C 含量为 41.3mol%。基于表型、基因型和系统发育分析，ORL-24(T) 菌株代表肠球菌属的一个新种，命名为肠球菌 diestrammenae。其模式菌株为 ORL-24(T)（=KACC 16708(T)=JCM 18359(T)）。
