Deguchi Y, Kishimoto S
Third Department of Internal Medicine, Osaka University School of Medicine, Japan.
Clin Rheumatol. 1990 Jun;9(2):182-5. doi: 10.1007/BF02031966.
In the present study, we show the spontaneous elevation of the transcription of heat shock protein (hsp 70) gene in peripheral blood mononuclear cells (PBMC) of individuals with active systemic lupus erythematosus (SLE) by nuclear run-on transcription assay. For quantification analysis of hsp 70 gene transcription, we first found more than ten times of the enhanced transcription of hsp 70 gene in PBMC of individuals with active SLE, as compared to normal healthy subjects or patients with bronchial asthma as controls. Our observation suggests that heat shock proteins may be produced during an active immune response in individuals with active systemic lupus erythematosus and is involved in lupus-related change of possible essential function in PBMC.
在本研究中，我们通过核转录分析表明，活动期系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中热休克蛋白（hsp 70）基因转录会自发升高。为了对hsp 70基因转录进行定量分析，我们首先发现，与正常健康受试者或作为对照的支气管哮喘患者相比，活动期SLE患者PBMC中hsp 70基因转录增强了十多倍。我们的观察结果表明，热休克蛋白可能在活动期系统性红斑狼疮患者的活跃免疫反应过程中产生，并参与PBMC中可能具有重要功能的狼疮相关变化。
