The Key Laboratory of Resource Chemistry of Ministry of Education, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, China.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):3583-91. doi: 10.1016/j.msec.2013.04.021. Epub 2013 Apr 13.
Implant-associated infection is a serious problem in orthopaedic surgery. One of the most effective ways is to introduce a controlled antibiotics delivery system into the bone filling materials, achieving sustained release of antibiotics in the local sites of bone defects. In the present work, mesoporous carbonated hydroxyapatite microspheres (MCHMs) loaded with gentamicin have been fabricated according to the following stages: (i) the preparation of the MCHMs by hydrothermal method using calcium carbonate microspheres as sacrificial templates, and (ii) loading gentamicin into the MCHMs. The MCHMs exhibit the 3D hierarchical nanostructures constructed by nanoplates as building blocks with mesopores and macropores, which make them have the higher drug loading efficiency of 70-75% than the conventional hydroxyapatite particles (HAPs) of 20-25%. The gentamicin-loaded MCHMs display the sustained drug release property, and the controlled release of gentamicin can minimize significantly bacterial adhesion and prevent biofilm formation against S. epidermidis. The biocompatibility tests by using human bone marrow stromal cells (hBMSCs) as cell models indicate that the gentamicin-loaded MCHMs have as excellent biocompatibility as the HAPs, and the dose of the released gentamicin from the MCHMs has no toxic effects on the hBMSCs. Hence, the gentamicin-loaded MCHMs can be served as a simple, non-toxic and controlled drug delivery system to treat bone infections.
植入物相关感染是骨科手术中的一个严重问题。其中最有效的方法之一是将控制抗生素释放系统引入骨填充材料中，在骨缺损的局部部位实现抗生素的持续释放。在本工作中，根据以下阶段制备载有庆大霉素的介孔碳酸羟基磷灰石微球（MCHMs）：（i）通过水热法使用碳酸钙微球作为牺牲模板制备 MCHMs，和（ii）将庆大霉素载入 MCHMs 中。MCHMs 呈现由纳米板构建的 3D 分级纳米结构，具有介孔和大孔，这使得它们的药物载量效率比传统的羟基磷灰石颗粒（HAPs）高 70-75%，而 HAPs 的药物载量效率为 20-25%。载庆大霉素的 MCHMs 表现出持续的药物释放性能，庆大霉素的控制释放可以显著减少表皮葡萄球菌的细菌黏附和生物膜形成。以人骨髓基质细胞（hBMSCs）作为细胞模型进行的生物相容性测试表明，载庆大霉素的 MCHMs 与 HAPs 具有优异的生物相容性，并且从 MCHMs 释放的庆大霉素的剂量对 hBMSCs 没有毒性作用。因此，载庆大霉素的 MCHMs 可用作一种简单、无毒且可控的药物释放系统，以治疗骨感染。
