Tarim University, Alar, Xinjiang, People's Republic of China.
Antonie Van Leeuwenhoek. 2010 Nov;98(4):447-53. doi: 10.1007/s10482-010-9458-9. Epub 2010 Jun 4.
A novel halophilic, filamentous actinobacterium, designated TRM 40136(T), was isolated from a hypersaline habitat in Xinjiang Province, north-west China. The strain is aerobic, Gram-positive, halophilic, and the optimum NaCl concentration for growth is 10-15% (w/v). The whole-cell sugar pattern consists of xylose, glucose and arabinose. The predominant menaquinone is MK-6 (51.2%) and the major fatty acids are anteiso-C15:0 (35.2%), anteiso-C17:0 (15.9%) and iso-C15:0 (13.7%). The phospholipid pattern consists of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and two unknown phospholipids. The G + C content of the genomic DNA is 68.9 mol%. Phylogenetic analysis showed that strain TRM 40136(T) had 16S rRNA gene sequence similarity of 96.1% with the closest described species Actinopolyspora mortivallis, and it can be distinguished from all species in the genus Actinopolyspora by using these data of polyphasic taxonomy study. On the basis of the polyphasic evidence, the strain TRM 40136(T) should be designated as a novel species of the genus Actinopolyspora for which the name Actinopolyspora xinjiangensis sp.nov. is proposed. The type strain is TRM 40136(T) (=CCTCC AA 209080(T) = KCTC 19656(T)).
一株新型嗜盐、丝状放线菌，命名为 TRM 40136(T)，从中国西北部新疆的一个高盐生境中分离得到。该菌株为需氧、革兰氏阳性、嗜盐，生长的最适 NaCl 浓度为 10-15%（w/v）。全细胞糖模式由木糖、葡萄糖和阿拉伯糖组成。主要的类异戊二烯醌为 MK-6（51.2%），主要脂肪酸为 anteiso-C15:0（35.2%）、anteiso-C17:0（15.9%）和 iso-C15:0（13.7%）。磷脂模式由磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱和两种未知磷脂组成。基因组 DNA 的 G+C 含量为 68.9 mol%。系统发育分析表明，菌株 TRM 40136(T)与最接近的描述种 Actinopolyspora mortivallis 的 16S rRNA 基因序列相似性为 96.1%，并且可以通过使用这些多相分类学研究的数据与放线菌属中的所有种区分开来。基于多相证据，菌株 TRM 40136(T)应被指定为放线菌属的一个新种，命名为 Actinopolyspora xinjiangensis sp.nov.。模式株为 TRM 40136(T)（=CCTCC AA 209080(T)=KCTC 19656(T)）。
