Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Bani-Suef University, Alshaheed Shehata Ahmad Hegazy St, 62111, Beni-Suef, Egypt,
J Fluoresc. 2014 Jan;24(1):129-35. doi: 10.1007/s10895-013-1276-9. Epub 2013 Aug 3.
Methocarbamol is formulated with Ibuprofen for treatment of alleviated pain associated with muscle spasm. This manuscript describes a sensitive and selective first derivative synchronous spectrofluorimetric method for simultaneous determination of both drugs. Factors affecting method selectivity were studied where best results were obtained upon using Δ λ = 20 and water as a solvent. Methocartbamol was determined at 283 nm while Ibuprofen at 285.5 nm in the concentration ranges of 0.4-5 and 0.2-4.8 μg/mL, respectively. The applicability of the proposed method was ascertained by application to different laboratory prepared mixtures and marketed formulation. The high sensitivity achieved by the proposed method permitted its application for determination of the drugs in human plasma spiked with pure drugs and their combined tablets. The proposed method showed no significant difference when compared with the reported HPLC method using student's t-test and F-ratio test.
美索巴莫与布洛芬联合用于治疗缓解肌肉痉挛相关的疼痛。本文描述了一种灵敏且选择性的一阶导数同步荧光光谱法，可同时测定这两种药物。对影响方法选择性的因素进行了研究，结果表明，当 Δλ=20，溶剂用水时，可获得最佳结果。在 0.4-5 和 0.2-4.8 μg/mL 的浓度范围内，美索巴莫在 283nm 处测定，布洛芬在 285.5nm 处测定。通过应用于不同实验室制备的混合物和市售制剂，确定了所提出方法的适用性。所提出的方法具有高灵敏度，可用于测定人血浆中加入纯药物和联合片剂后药物的含量。通过学生 t 检验和 F 检验，与已报道的 HPLC 方法相比，该方法没有显著差异。
