体外研究荜茇和荜澄茄提取物对口腔念珠菌黏附的抑制作用。

An in vitro study on the anti-adherence effect of Brucea javanica and Piper betle extracts towards oral Candida.

机构信息

Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.

出版信息

Arch Oral Biol. 2013 Oct;58(10):1335-42. doi: 10.1016/j.archoralbio.2013.07.001. Epub 2013 Jul 31.

DOI:10.1016/j.archoralbio.2013.07.001

PMID:23915676

Abstract

OBJECTIVE

The adherence of Candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. The study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida.

METHODS

In the former, adsorption to hexadecane was used to measure the hydrophobic interaction of the candida cells. In the later, glass beads coated with saliva represented the experimental pellicles in specific adhesion of oral candida to hard tissue surface.

RESULTS

Candida krusei, Candida dubliniensis and Candida tropicalis showed the highest adsorption to hexadecane at 30.23%, 26.19% and 19.70%, respectively, while the others within the range of 7-10%. All candidal species were significantly affected by the extracts (P<0.05) with Brucea javanica exhibited more than 60% reduction of CSH than Piper betle. Candida parapsilosis showed the highest affinity in specific-bindings to pellicle with 18.72±0.71×10(5)CFU/ml. Exposing to P. betle-treated pellicle has drastically reduced the adherence of C. tropicalis, Candida albicans and C. krusei by 86.01%, 61.41% and 56.34%, respectively. B. javanica exhibited similar effect on C. tropicalis (89.86%), Candida lusitaniae (88.95%), C. albicans (79.74%), Candida glabrata (76.85%) and C. krusei (67.61%).

CONCLUSION

The extracts demonstrated anti-adherence activities by modifying the CSH and the characteristics of the experimental pellicle.

摘要

目的

白色念珠菌黏附于黏膜表面是其成功入侵口腔的初始步骤。本研究旨在探究两种植物提取物对口腔白色念珠菌非特异性和特异性黏附的影响。

方法

前者采用十六烷吸附法测量白色念珠菌细胞的疏水性相互作用。后者中，涂覆唾液的玻璃珠代表白色念珠菌在硬组织表面的特异性黏附的实验菌膜。

结果

Candida krusei、Candida dubliniensis 和 Candida tropicalis 对十六烷的吸附率分别高达 30.23%、26.19%和 19.70%，而其他菌株的吸附率则在 7-10%范围内。所有白色念珠菌的吸附率均受到提取物的显著影响（P<0.05），其中荜茇提取物对 CSH 的降低作用超过 60%，荜澄茄提取物的作用大于胡椒提取物。在特异性黏附实验中，Candida parapsilosis 对菌膜的亲和力最高，达到 18.72±0.71×10(5)CFU/ml。暴露于荜澄茄处理的菌膜后，C. tropicalis、Candida albicans 和 C. krusei 的黏附率分别大幅降低了 86.01%、61.41%和 56.34%。荜茇提取物对 C. tropicalis（89.86%）、Candida lusitaniae（88.95%）、Candida albicans（79.74%）、Candida glabrata（76.85%）和 C. krusei（67.61%）也表现出相似的抑制效果。