Department of Oral Biology, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2013;2013:397268. doi: 10.1155/2013/397268. Epub 2013 Jun 18.
The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts. All candidal suspensions were standardized to 1 × 10(6) cells/mL. The suspension was incubated overnight at 37 °C (C. parapsilosis, 35°C). Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1. HWP1 mRNAs were only detected in C. albicans, C. parapsilosis, and C. tropicalis. Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts. C. albicans, C. parapsilosis, and C. tropicalis have demonstrated different intensity of mRNA. Compared to P. betle, B. javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples. HWP1 was not detected in C. albicans following the treatment of B. javanica at 1 mg/mL. In contrast, C. parapsilosis and C. tropicalis were shown to have HWP1 regulation. However, the expression levels were reduced upon the addition of higher concentration of B. javanica extract. P. betle and B. javanica have potential to be developed as oral health product.
本研究旨在鉴定非白念珠菌属念珠菌中的 HWP1 基因，以及在胡椒和鸦胆子水提物处理后 HWP1 的差异表达。将所有念珠菌悬浮液标准化至 1×10(6)个细胞/ml。将悬浮液在 37°C（近平滑念珠菌为 35°C）下孵育过夜。用各提取物以 1、3 和 6mg/ml 处理念珠菌细胞 24h。提取总 RNA，并用 HWP1 特异性引物进行逆转录聚合酶链反应。仅在白念珠菌、近平滑念珠菌和热带念珠菌中检测到 HWP1 mRNAs。暴露于水提取物会影响 HWP1 转录物的表达。白念珠菌、近平滑念珠菌和热带念珠菌的 mRNA 表达强度不同。与胡椒相比，鸦胆子在所有样本中对 HWP1 转录水平的抑制作用更高。在 1mg/ml 鸦胆子处理后，白念珠菌中未检测到 HWP1。相比之下，近平滑念珠菌和热带念珠菌表现出 HWP1 的调控。然而，随着鸦胆子提取物浓度的增加，表达水平降低。胡椒和鸦胆子有可能开发为口腔保健产品。
