Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, Canada.
Sci Rep. 2013;3:2366. doi: 10.1038/srep02366.
Present study, for the first time, reports the development of a nanohybridized baculovirus based stent that can locally promote vascular re-endothelialization by efficient delivery of pro-angiogenic vascular endothelial growth factor (Vegf) genes. In vitro data demonstrated rapid expression of functionally active Vegf by the bioactive stent-transduced vascular cells. In vivo site-specific transgene expression was observed at the stented regions of balloon-denuded canine femoral artery, which eventually lead to significant endothelial recovery at the injured sites. A significant reduction in neointima formation (2.23 ± 0.56 mm(2) vs 2.78 ± 0.49 mm(2) and 3.11 ± 0.23 mm(2), p < 0.05; n = 8) and percent stenosis was observed in treated stent group compared to negative control and bare metal stent groups. These findings collectively implicate the potential of this newly developed baculovirus based biotherapeutic stent to ameliorate damaged vascular biology and attenuate re-narrowing of stented artery by inhibiting neointima formation.
本研究首次报道了一种基于纳米杂交杆状病毒的支架的开发，该支架可通过高效递送电活性血管内皮生长因子 (Vegf) 基因，局部促进血管再内皮化。体外数据表明，生物活性支架转导的血管细胞可快速表达具有功能活性的 Vegf。在球囊去内皮犬股动脉支架部位观察到了部位特异性的转基因表达，最终导致了损伤部位的内皮显著恢复。与阴性对照组和裸金属支架组相比，治疗支架组的新生内膜形成（2.23 ± 0.56 mm(2) 比 2.78 ± 0.49 mm(2) 和 3.11 ± 0.23 mm(2)，p < 0.05；n = 8）和狭窄百分比明显降低。这些发现共同表明，这种新开发的基于杆状病毒的生物治疗性支架具有改善受损血管生物学和抑制新生内膜形成来减轻支架动脉再狭窄的潜力。
