Santisukwongchote Kobchai, Amornlertwatana Yutti, Sastraruji Thanapat, Jaikang Churdsak
Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Excellence in Oral and Maxillofacial Biology, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand.
Metabolites. 2019 Dec 19;10(1):6. doi: 10.3390/metabo10010006.
Coronary heart disease (CHD) is the major cause of death in sudden unexpected death (SUD) cases. Tryptophan (TRP) and its metabolites are correlated with the CHD patient but less studies in the SUD. The aim of this study was to evaluate the relationship of TRP and its metabolites with the CHD in the SUD cases. Blood samples and heart tissues were collected from CHD subjects ( = 31) and the control group ( = 72). Levels of kynurenine (KYN), kynurenic acid (KYA), xanthurenic acid (XAN), 3-hydroxyanthranillic acid (HAA), quinolinic acid (QA), picolinic acid (PA) and 5-hydroxyindoleacetic acid (HIAA) were determined by HPLC-DAD. A severity of heart occlusion was categorized into four groups, and the relationship was measured with the TRP metabolites. The HIAA and The KYN levels significantly differed ( < 0.01) between the CHD group and the control group. Lower levels of QA/XAN, PA/KA, HAA/XAN, KYN/XAN and KYN/TRP were found in the CHD group. However, PA/HAA, PA/HIAA, PA/KYN and XAN/KA values in the CHD group were higher than the control group ( < 0.05). This study revealed that the values of PA/KA and PA/HAA provided better choices for a CHD biomarker in postmortem bodies.
冠心病（CHD）是意外猝死（SUD）病例的主要死因。色氨酸（TRP）及其代谢产物与冠心病患者相关，但在SUD方面的研究较少。本研究的目的是评估TRP及其代谢产物与SUD病例中冠心病的关系。从冠心病受试者（n = 31）和对照组（n = 72）采集血液样本和心脏组织。通过高效液相色谱 - 二极管阵列检测法（HPLC - DAD）测定犬尿氨酸（KYN）、犬尿酸（KYA）、黄尿酸（XAN）、3 - 羟基邻氨基苯甲酸（HAA）、喹啉酸（QA）、吡啶酸（PA）和5 - 羟基吲哚乙酸（HIAA）的水平。将心脏阻塞的严重程度分为四组，并测量与TRP代谢产物的关系。CHD组和对照组之间的HIAA和KYN水平存在显著差异（P < 0.01）。在CHD组中发现QA/XAN、PA/KA、HAA/XAN、KYN/XAN和KYN/TRP的水平较低。然而，CHD组中的PA/HAA、PA/HIAA、PA/KYN和XAN/KA值高于对照组（P < 0.05）。本研究表明，PA/KA和PA/HAA的值为死后尸体中冠心病生物标志物提供了更好的选择。
