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自体骨移植中松质骨与皮质骨界面的组织计量学分析。

Histometric analyses of cancellous and cortical interface in autogenous bone grafting.

作者信息

Netto Henrique Duque, Olate Sergio, Klüppel Leandro, do Carmo Antonio Marcio Resende, Vásquez Bélgica, Albergaria-Barbosa Jose

机构信息

Dental School, Federal University of Juiz de Fora, Juiz de Fora, Brazil.

出版信息

Int J Clin Exp Pathol. 2013 Jul 15;6(8):1532-7. Print 2013.

Abstract

Surgical procedures involving the rehabilitation of the maxillofacial region frequently require bone grafts; the aim of this research was to evaluate the interface between recipient and graft with cortical or cancellous contact. 6 adult beagle dogs with 15 kg weight were included in the study. Under general anesthesia, an 8 mm diameter block was obtained from parietal bone of each animal and was put on the frontal bone with a 12 mm 1.5 screws. Was used the lag screw technique from better contact between the recipient and graft. 3-week and 6-week euthanized period were chosen for histometric evaluation. Hematoxylin-eosin was used in a histologic routine technique and histomorphometry was realized with IMAGEJ software. T test was used for data analyses with p<0.05 for statistical significance. The result show some differences in descriptive histology but non statistical differences in the interface between cortical or cancellous bone at 3 or 6 week; as natural, after 6 week of surgery, bone integration was better and statistically superior to 3-week analyses. We conclude that integration of cortical or cancellous bone can be usefully without differences.

摘要

涉及颌面区域修复的外科手术常常需要骨移植;本研究的目的是评估接受骨与具有皮质骨或松质骨接触的移植骨之间的界面。该研究纳入了6只体重15千克的成年比格犬。在全身麻醉下,从每只动物的顶骨获取一个直径8毫米的骨块,并用12毫米的1.5螺钉将其固定在额骨上。采用拉力螺钉技术以实现接受骨与移植骨之间的更好接触。选择3周和6周的安乐死期进行组织计量学评估。在组织学常规技术中使用苏木精-伊红染色,并用IMAGEJ软件进行组织形态计量学分析。采用t检验进行数据分析,p<0.05具有统计学意义。结果显示在描述性组织学上存在一些差异,但在3周或6周时皮质骨或松质骨界面无统计学差异;自然地,术后6周时骨整合更好,且在统计学上优于3周分析。我们得出结论,皮质骨或松质骨的整合效果良好,无差异。

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