Oxford University Clinical Research Unit- Patan Hospital, Nepal.
Sci Rep. 2013;3:2382. doi: 10.1038/srep02382.
We conducted a prospective hospital based study from February 2009-April 2011 to identify the possible pathogens of central nervous system (CNS) infections in adults admitted to a tertiary referral hospital (Patan Hospital) in Kathmandu, Nepal. The pathogens of CNS infections were confirmed in cerebrospinal fluid (CSF) using molecular diagnostics, culture (bacteria) and serology. 87 patients were recruited for the study and the etiological diagnosis was established in 38% (n = 33). The bacterial pathogens identified were Neisseria meningitidis (n = 6); Streptococcus pneumoniae (n = 5) and Staphylococcus aureus (n = 2) in 13/87(14%). Enteroviruses were found in 12/87 (13%); Herpes Simplex virus (HSV) in 2/87(2%). IgM against Japanese encephalitis virus (JEV) was detected in the CSF of 11/73 (15%) tested samples. This is the first prospective molecular and serology based CSF analysis in adults with CNS infections in Kathmandu, Nepal. JEV and enteroviruses were the most commonly detected pathogens in this setting.
我们于 2009 年 2 月至 2011 年 4 月进行了一项前瞻性的基于医院的研究，以确定在尼泊尔加德满都的一家三级转诊医院（巴丹医院）收治的成人中枢神经系统 (CNS) 感染的可能病原体。使用分子诊断、培养（细菌）和血清学方法在脑脊液 (CSF) 中确认了 CNS 感染的病原体。本研究共招募了 87 名患者，确定了 38%（n=33）的病因诊断。在 87 名患者中鉴定出的细菌病原体包括脑膜炎奈瑟菌 (n=6)；肺炎链球菌 (n=5)和金黄色葡萄球菌 (n=2)，共 13/87(14%)。12/87 (13%)发现肠道病毒；2/87(2%)发现单纯疱疹病毒 (HSV)。在 73 份送检 CSF 样本中，有 11/73(15%)检测到乙型脑炎病毒 (JEV)的 IgM。这是在尼泊尔加德满都首次对成人中枢神经系统感染进行基于前瞻性分子和血清学的 CSF 分析。在这种情况下，JEV 和肠道病毒是最常检测到的病原体。
