Farideh Zafari Zangeneh, Bagher Minaee, Ashraf Amirzargar, Akram Ahangarpour, Kazem Mousavizadeh
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran.
J Reprod Infertil. 2010 Oct;11(3):169-74.
Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder associated with ovulatory dysfunction. Presently, little is known about the primary factors that initiate PCOS. Chamomile flowers are used in alternative medicine for its anti-spasmolytic and anti-inflammatory effects. Antispasmodic properties of chamomile ease menstrual cramps and lessen the possibility of premature labor. This medicinal herb also stimulates menstruation. In this study, we evaluated the effects of Chamomile alcoholic-extract on the biochemical and clinical parameters in a rat model of PCOS.
Estrous cyclicity of 30 virgin adult cycling rats was monitored by vaginal smears obtained between 0800 and 1200 hours. After about 4 days, each rat received an i.m. injection of Estradiol Valerate (Aburaihan Co., Iran), 2 mg in 0.2 ml of corn oil, to induce PCO. Corn oil was injected to the rats in the control group. All the rats in the experimental group were evaluated for follicular cysts 60 days after the injection. Rats with PCOS were treated by multiple doses (25, 50, 75 mg/kg) of intraperitoneal injections of Chamomile alcoholic-extract for ten days. The data were statistically analyzed at a significance level of p<0.05 by ANOVA, followed by the Student Newman-Keuls post hoc test.
The histological and hormonal results showed that Chamomile can decrease the signs of PCOS in the ovarian tissue and help LH secretion in rats (p<0.05).
The alcoholic-extract of dried Matricaria chamomilla L. flowers can not only induce recovery from a PCO induced state in rats, but also increase dominant follicles. Additionally better endometrial tissue arrangements can be regarded as another therapeutic effect of Chamomile.
多囊卵巢综合征（PCOS）是一种与排卵功能障碍相关的复杂内分泌和代谢紊乱疾病。目前，对于引发PCOS的主要因素知之甚少。洋甘菊花因其解痉和抗炎作用而被用于替代医学。洋甘菊的解痉特性可缓解痛经并降低早产的可能性。这种草药还能刺激月经。在本研究中，我们评估了洋甘菊醇提物对PCOS大鼠模型生化和临床参数的影响。
通过在08:00至12:00之间采集的阴道涂片监测30只成年未孕发情周期大鼠的发情周期。约4天后，每只大鼠肌肉注射戊酸雌二醇（伊朗阿卜赖汉公司），2mg溶于0.2ml玉米油中，以诱导多囊卵巢（PCO）。对照组大鼠注射玉米油。注射后60天对实验组所有大鼠进行卵泡囊肿评估。对患有PCOS的大鼠腹腔注射多剂量（25、50、75mg/kg）洋甘菊醇提物，持续十天。数据采用方差分析进行统计学分析，显著性水平为p<0.05，随后进行Student Newman-Keuls事后检验。
组织学和激素结果表明，洋甘菊可减轻大鼠卵巢组织中PCOS的症状，并有助于大鼠促黄体生成素（LH）的分泌（p<0.05）。
干母菊花的醇提物不仅能使大鼠从PCO诱导状态恢复，还能增加优势卵泡。此外，更好的子宫内膜组织排列可被视为洋甘菊的另一种治疗效果。
