Universidade Federal do Rio de Janeiro, Programa de Biotecnologia Vegetal, CCS, Bl. K, Rio de Janeiro, RJ, Brazil.
Phytochemistry. 2010 Feb;71(2-3):294-300. doi: 10.1016/j.phytochem.2009.10.007. Epub 2009 Nov 16.
The flavone glycosides, named scutellarein-7-O-beta-D-apiofuranoside and apigenin-7-O-beta-D-apiofuranosyl-(1-->2)-beta-D-apiofuranoside, and the flavone celtidifoline (5,6,4',5'-tetrahydroxy-7,3'-dimethoxyflavone), along with other 11 known compounds, were isolated from leaves of the ethyl acetate extract of Lantana trifolia L. using step gradient High Speed Countercurrent Chromatography (HSCCC) and High Performance Liquid Chromatography (HPLC), respectively. Their structures were elucidated by spectroscopic methods, including 2D NMR and mass spectrometry (ESI-MS) techniques. The ethanolic and ethyl acetate extracts produced an intense sedative effect in mice, one hour after oral administration of 1 mg/kg. This effect was neither due to a benzodiazepine-like effect of the three flavone derivatives neither of the phenylpropanoids, betonyoside F and verbascoside, that were tested for their affinity for the [3H] flunitrazepam binding sites.
从三叶鬼针草叶的乙酸乙酯提取物中，分别采用分步梯度高速逆流色谱（HSCCC）和高效液相色谱（HPLC），分离得到两种黄酮苷类化合物，即野黄芩苷-7-O-β-D-芹糖基-(1→2)-β-D-芹糖苷和芹菜素-7-O-β-D-芹糖基-(1→2)-β-D-芹糖苷，以及另一种黄酮化合物 Celtidifoline（5,6,4',5'-四羟基-7,3'-二甲氧基黄酮），同时还分离得到了 11 种已知化合物。通过光谱方法，包括二维 NMR 和质谱（ESI-MS）技术，鉴定了它们的结构。在给予 1mg/kg 口服后 1 小时，乙醇和乙酸乙酯提取物对小鼠产生了强烈的镇静作用。这种作用既不是由于三种黄酮衍生物的苯丙素类化合物的苯并二氮䓬样作用引起的，也不是由于测试其对 [3H]氟硝西泮结合位点亲和力的贝托尼苷 F 和维斯巴苷引起的。
