双酚 A 通过芳香烃受体信号通路部分抑制培养的小鼠卵巢卵泡生长。
Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway.
机构信息
Department of Comparative Biosciences, University of Illinois, Urbana, IL, USA.
出版信息
Reprod Toxicol. 2013 Dec;42:58-67. doi: 10.1016/j.reprotox.2013.07.022. Epub 2013 Aug 6.
Abstract
Bisphenol A (BPA) is an endocrine disruptor that inhibits growth of mouse ovarian follicles and disrupts steroidogenesis at a dose of 438μM. However, the effects of lower doses of BPA and its mechanism of action in ovarian follicles are unknown. We hypothesized that low doses of BPA inhibit follicular growth and decrease estradiol levels through the aryl hydrocarbon receptor (AHR) pathway. Antral follicles from wild-type and Ahr knock-out (AhrKO) mice were cultured for 96h. Follicle diameters and estradiol levels then were compared in wild-type and AhrKO follicles ± BPA (0.004-438μM). BPA inhibited follicle growth (110-438μM) and decreased estradiol levels (43.8-438μM) in wild-type and AhrKO follicles. However, at BPA 110μM, inhibition of growth in AhrKO follicles was attenuated compared to wild-type follicles. These data suggest that BPA may inhibit follicle growth partially via the AHR pathway, whereas its effects on estradiol synthesis likely involve other mechanisms.
摘要
双酚 A（BPA）是一种内分泌干扰物，在 438μM 的剂量下会抑制小鼠卵巢卵泡的生长并破坏类固醇生成。然而，较低剂量的 BPA 的影响及其在卵巢卵泡中的作用机制尚不清楚。我们假设低剂量的 BPA 通过芳香烃受体（AHR）途径抑制卵泡生长并降低雌二醇水平。从野生型和 Ahr 敲除（AhrKO）小鼠中培养出腔前卵泡，并培养 96 小时。然后比较野生型和 AhrKO 卵泡中有无 BPA（0.004-438μM）时的卵泡直径和雌二醇水平。BPA 抑制了野生型和 AhrKO 卵泡的卵泡生长（110-438μM）并降低了雌二醇水平（43.8-438μM）。然而，在 BPA 110μM 时，AhrKO 卵泡的生长抑制作用比野生型卵泡减弱。这些数据表明，BPA 可能部分通过 AHR 途径抑制卵泡生长，而其对雌二醇合成的影响可能涉及其他机制。
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