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本文引用的文献

1
The safe use of a PTEN inhibitor for the activation of dormant mouse primordial follicles and generation of fertilizable eggs.PTEN 抑制剂安全激活休眠的小鼠原始卵泡并产生可受精的卵子。
PLoS One. 2012;7(6):e39034. doi: 10.1371/journal.pone.0039034. Epub 2012 Jun 27.
2
Designing cell-compatible hydrogels for biomedical applications.设计用于生物医学应用的细胞相容性水凝胶。
Science. 2012 Jun 1;336(6085):1124-8. doi: 10.1126/science.1214804.
3
Effect of cryopreservation and transplantation on the expression of kit ligand and anti-Mullerian hormone in human ovarian tissue.冷冻保存和移植对人卵巢组织中 kit 配体和抗苗勒管激素表达的影响。
Hum Reprod. 2012 Apr;27(4):1088-95. doi: 10.1093/humrep/des013. Epub 2012 Feb 7.
4
Ovary cryopreservation and transplantation for fertility preservation.卵巢组织冻存与移植用于保存生育力。
Mol Hum Reprod. 2012 Feb;18(2):59-67. doi: 10.1093/molehr/gar082. Epub 2011 Dec 28.
5
Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles.用于冷冻解冻人卵巢皮质卵泡器官培养的藻酸盐支架。
J Assist Reprod Genet. 2011 Sep;28(9):761-9. doi: 10.1007/s10815-011-9605-x. Epub 2011 Jul 23.
6
Neonatal exposure to bisphenol A or diethylstilbestrol alters the ovarian follicular dynamics in the lamb.新生期暴露于双酚 A 或己烯雌酚会改变羔羊的卵巢卵泡动态。
Reprod Toxicol. 2011 Nov;32(3):304-12. doi: 10.1016/j.reprotox.2011.06.118. Epub 2011 Jun 21.
7
Growth differentiating factor 9 (GDF9) and bone morphogenetic protein 15 both activate development of human primordial follicles in vitro, with seemingly more beneficial effects of GDF9.生长分化因子 9（GDF9）和骨形态发生蛋白 15 均可在体外激活人原始卵泡的发育，其中 GDF9 的作用似乎更为有利。
J Clin Endocrinol Metab. 2011 Aug;96(8):E1246-54. doi: 10.1210/jc.2011-0410. Epub 2011 Jun 1.
8
Micro-organ ovarian transplantation enables pregnancy: a case report.卵巢组织微移植实现妊娠：病例报告。
Hum Reprod. 2011 May;26(5):1097-103. doi: 10.1093/humrep/der063. Epub 2011 Mar 18.
9
Activation of dormant ovarian follicles to generate mature eggs.激活休眠的卵巢卵泡以产生成熟卵子。
Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10280-4. doi: 10.1073/pnas.1001198107. Epub 2010 May 17.
10
Occasional involvement of the ovary in Ewing sarcoma.卵巢偶尔会被尤因肉瘤累及。
Hum Reprod. 2010 Jul;25(7):1708-12. doi: 10.1093/humrep/deq121. Epub 2010 May 15.

尝试将具有增强型三磷酸肌醇的生物工程/生物合成支撑基质应用于人原始卵泡的器官培养。

Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles.

机构信息

Infertility and IVF Unit, Beilinson Hospital for Women, Rabin Medical Center, Petach Tikva, 49100, Israel.

出版信息

J Assist Reprod Genet. 2013 Oct;30(10):1279-88. doi: 10.1007/s10815-013-0052-8. Epub 2013 Aug 11.

DOI:10.1007/s10815-013-0052-8
PMID:23934019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3824864/
Abstract

PURPOSE

To improve human primordial follicle culture.

METHODS

Thin or thick ovarian slices were cultured on alginate scaffolds or in PEG-fibrinogen hydrogels with or without bpV (pic), which prevents the conversion of phosphatidylinositol-trisphosphate (PIP3) to phosphatidylinositol-bisphosphate (PIP2) or 740Y-P which converts PIP2 to PIP3. Follicular growth was evaluated by follicular counts, Ki67 immunohistochemistry, and 17β-estradiol (E2) levels.

RESULTS

BpV (pic) had a destructive effect on cultured follicles. Thawed-uncultured samples had more primordial follicles than samples cultured in basic medium and fewer developing follicles than samples cultured in PEG-fibrinogen hydrogels with 740Y-P. There were more atretic follicles in samples cultured on alginate scaffolds than in PEG-fibrinogen hydrogels, and in samples cultured in PEG-fibrinogen hydrogels with 740Y-P than in PEG-fibrinogen hydrogels with basic medium. Ki67 staining was higher in PEG-fibrinogen hydrogels than on alginate scaffolds. E2 levels were higher in thick than in thin slices.

CONCLUSIONS

PEG-fibrinogen hydrogels appear to have an advantage over alginate scaffolds for culturing human primordial follicles. Folliculogenesis is not increased in the presence of substances that enhance PIP3 production or with thin rather than thick sectioning.

摘要

目的

提高人类原始卵泡培养效果。

方法

将薄或厚的卵巢切片置于藻酸盐支架上或聚乙二醇-纤维蛋白原水凝胶中培养，这些水凝胶中加入了 bpV（pic）或 740Y-P，前者可阻止磷脂酰肌醇三磷酸（PIP3）转化为磷脂酰肌醇二磷酸（PIP2），后者可将 PIP2 转化为 PIP3。通过卵泡计数、Ki67 免疫组化和 17β-雌二醇（E2）水平评估卵泡生长情况。

结果

bpV（pic）对培养的卵泡具有破坏性影响。与在基础培养基中培养的样本相比，解冻未培养的样本具有更多的原始卵泡，而与在含有 740Y-P 的聚乙二醇-纤维蛋白原水凝胶中培养的样本相比，发育中的卵泡更少。与聚乙二醇-纤维蛋白原水凝胶相比，在藻酸盐支架上培养的样本中，有更多的闭锁卵泡，而在含有 740Y-P 的聚乙二醇-纤维蛋白原水凝胶中培养的样本比在含有基础培养基的聚乙二醇-纤维蛋白原水凝胶中培养的样本更多。Ki67 染色在聚乙二醇-纤维蛋白原水凝胶中比在藻酸盐支架上更高。E2 水平在厚切片中高于薄切片。

结论

聚乙二醇-纤维蛋白原水凝胶似乎比藻酸盐支架更有利于培养人类原始卵泡。在促进 PIP3 产生的物质存在下，或使用较薄而不是较厚的切片时，卵泡发生并未增加。