• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过血清蛋白质组学分析鉴定膝骨关节炎的潜在预后标志物

Identification of potential prognostic markers for knee osteoarthritis by serum proteomic analysis.

作者信息

Takinami Yoshihiko, Yoshimatsu Shinya, Uchiumi Takaoki, Toyosaki-Maeda Tomoko, Morita Atsushi, Ishihara Takeshi, Yamane Shoji, Fukuda Isao, Okamoto Hiroyuki, Numata Yoshito, Fukui Naoshi

机构信息

Shionogi Pharmaceutical Research Center, Shionogi and Co., Ltd., Osaka, Japan.

出版信息

Biomark Insights. 2013 Jul 29;8:85-95. doi: 10.4137/BMI.S11966. Print 2013.

DOI:10.4137/BMI.S11966
PMID:23935359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3735238/
Abstract

BACKGROUND

As osteoarthritis (OA) is a highly heterogeneous disease in terms of progression, establishment of prognostic biomarkers would be highly beneficial for treatment. The present study was performed to identify novel biomarkers capable of predicting the progression of knee OA.

METHODS

A total of 69 plasma samples (OA patients undergoing radiographic progression, n = 25; nonprogression, n = 33; healthy donors, n = 11) were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), and ion peaks of interest were identified by liquid chromatography and matrix-assisted laser desorption/ionization (MALDI)-TOF MS. The identities of these proteins were further validated by immunoprecipitation combined with SELDI-TOF MS analysis.

RESULTS

SELDI-TOF MS analysis indicated that the intensities of 3 ion peaks differed significantly between progressors and nonprogressors. Subsequent analyses indicated that these peaks corresponded to apolipoprotein C-I, C-III, and an N-terminal truncated form of transthyretin, respectively. The identities of these proteins were confirmed by the loss of ion peaks in SELDI-TOF MS spectra by immunoprecipitation using specific antibodies for the respective proteins.

CONCLUSIONS

Three potential biomarkers were identified whose serum levels differed significantly between OA progressors and nonprogressors. These biomarkers are expected to be prognostic biomarkers for knee OA and to facilitate the development of novel disease-modifying treatments for OA.

摘要

背景

由于骨关节炎（OA）在疾病进展方面是一种高度异质性疾病，因此建立预后生物标志物将对治疗非常有益。本研究旨在识别能够预测膝关节OA进展的新型生物标志物。

方法

通过表面增强激光解吸/电离飞行时间质谱（SELDI-TOF MS）分析了总共69份血浆样本（经历影像学进展的OA患者，n = 25；无进展患者，n = 33；健康供体，n = 11），并通过液相色谱和基质辅助激光解吸/电离（MALDI）-TOF MS鉴定了感兴趣的离子峰。通过免疫沉淀结合SELDI-TOF MS分析进一步验证了这些蛋白质的身份。

结果

SELDI-TOF MS分析表明，进展者和无进展者之间3个离子峰的强度存在显著差异。随后的分析表明，这些峰分别对应载脂蛋白C-I、C-III和转甲状腺素蛋白的N端截短形式。通过使用针对各自蛋白质特异性抗体的免疫沉淀，SELDI-TOF MS光谱中离子峰的消失证实了这些蛋白质的身份。

结论

鉴定出三种潜在的生物标志物，其血清水平在OA进展者和无进展者之间存在显著差异。这些生物标志物有望成为膝关节OA的预后生物标志物，并有助于开发针对OA的新型疾病修饰治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/db0c547aa1bf/bmi-8-2013-085f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/5be620bf452d/bmi-8-2013-085f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/259d71f61910/bmi-8-2013-085f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/0d898bcb556a/bmi-8-2013-085f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/e59d3488b86a/bmi-8-2013-085f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/db0c547aa1bf/bmi-8-2013-085f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/5be620bf452d/bmi-8-2013-085f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/259d71f61910/bmi-8-2013-085f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/0d898bcb556a/bmi-8-2013-085f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/e59d3488b86a/bmi-8-2013-085f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7531/3735238/db0c547aa1bf/bmi-8-2013-085f5.jpg

相似文献

1
Identification of potential prognostic markers for knee osteoarthritis by serum proteomic analysis.通过血清蛋白质组学分析鉴定膝骨关节炎的潜在预后标志物
Biomark Insights. 2013 Jul 29;8:85-95. doi: 10.4137/BMI.S11966. Print 2013.
2
Analysis of synovial fluid in knee joint of osteoarthritis:5 proteome patterns of joint inflammation based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.骨关节炎膝关节滑液分析：基于基质辅助激光解吸电离飞行时间质谱的 5 种关节炎症蛋白质组模式。
Int Orthop. 2012 Jan;36(1):57-64. doi: 10.1007/s00264-011-1258-y. Epub 2011 Apr 21.
3
Identification of protein biomarkers in Dupuytren's contracture using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS).使用表面增强激光解吸电离飞行时间质谱法（SELDI-TOF-MS）鉴定杜普伊特伦挛缩症中的蛋白质生物标志物。
Clin Invest Med. 2006 Jun;29(3):136-45.
4
Potential plasma biomarkers for progression of knee osteoarthritis using glycoproteomic analysis coupled with a 2D-LC-MALDI system.使用糖蛋白质组学分析与 2D-LC-MALDI 系统联用，寻找膝关节骨关节炎进展的潜在血浆生物标志物。
Proteome Sci. 2012 Jun 6;10(1):36. doi: 10.1186/1477-5956-10-36.
5
A serum proteomic pattern for the detection of colorectal adenocarcinoma using surface enhanced laser desorption and ionization mass spectrometry.一种使用表面增强激光解吸电离质谱法检测结肠直肠癌的血清蛋白质组模式。
Cancer Invest. 2006 Dec;24(8):747-53. doi: 10.1080/07357900601063873.
6
Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer.通过质谱分析揭示的新型血清蛋白生物标志物组及其在乳腺癌中的预后价值。
Breast Cancer Res. 2014 Jun 16;16(3):R63. doi: 10.1186/bcr3676.
7
Quality control and quality assessment of data from surface-enhanced laser desorption/ionization (SELDI) time-of flight (TOF) mass spectrometry (MS).表面增强激光解吸/电离（SELDI）飞行时间（TOF）质谱（MS）数据的质量控制与质量评估。
BMC Bioinformatics. 2005 Jul 15;6 Suppl 2(Suppl 2):S5. doi: 10.1186/1471-2105-6-S2-S5.
8
Pancreatic cancer biomarkers discovery by surface-enhanced laser desorption and ionization time-of-flight mass spectrometry.通过表面增强激光解吸电离飞行时间质谱法发现胰腺癌生物标志物
Clin Chem Lab Med. 2009;47(6):713-23. doi: 10.1515/CCLM.2009.158.
9
Serum proteomics and biomarkers in hepatocellular carcinoma and chronic liver disease.肝细胞癌和慢性肝病中的血清蛋白质组学与生物标志物
Clin Cancer Res. 2008 Jan 15;14(2):470-7. doi: 10.1158/1078-0432.CCR-07-0586.
10
Identification of protein biomarkers for schizophrenia and bipolar disorder in the postmortem prefrontal cortex using SELDI-TOF-MS ProteinChip profiling combined with MALDI-TOF-PSD-MS analysis.利用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）蛋白质芯片分析结合基质辅助激光解吸电离飞行时间后源衰变质谱（MALDI-TOF-PSD-MS）分析，在死后前额叶皮质中鉴定精神分裂症和双相情感障碍的蛋白质生物标志物。
Neurobiol Dis. 2006 Jul;23(1):61-76. doi: 10.1016/j.nbd.2006.02.002. Epub 2006 Mar 20.

引用本文的文献

1
Proteomic profiling of serum in cats with naturally occurring degenerative joint disease and co-morbid conditions.对患有自然发生的退行性关节疾病及共病的猫的血清进行蛋白质组分析。
Front Pain Res (Lausanne). 2025 Feb 4;6:1501932. doi: 10.3389/fpain.2025.1501932. eCollection 2025.
2
Mass spectrometry-based proteomics identify novel serum osteoarthritis biomarkers.基于质谱的蛋白质组学鉴定新型血清骨关节炎生物标志物。
Arthritis Res Ther. 2022 May 23;24(1):120. doi: 10.1186/s13075-022-02801-1.
3
Quantitative proteomic analysis comparing grades ICRS1 and ICRS3 in patients with osteoarthritis.

本文引用的文献

1
Relationship between radiographic changes and symptoms or physical examination findings in subjects with symptomatic medial knee osteoarthritis: a three-year prospective study.症状性内侧膝关节骨关节炎患者的影像学改变与症状或体格检查结果的关系：一项为期三年的前瞻性研究。
BMC Musculoskelet Disord. 2010 Nov 24;11:269. doi: 10.1186/1471-2474-11-269.
2
Serum transforming growth factor-beta 1 is not a robust biomarker of incident and progressive radiographic osteoarthritis at the hip and knee: the Johnston County Osteoarthritis Project.血清转化生长因子-β1 不是髋关节和膝关节新发和进展性放射学骨关节炎的可靠生物标志物：约翰斯顿县骨关节炎项目。
Osteoarthritis Cartilage. 2010 Jun;18(6):825-9. doi: 10.1016/j.joca.2010.02.013. Epub 2010 Feb 17.
3
骨关节炎患者中 ICRS1 级和 ICRS3 级的定量蛋白质组学分析
Exp Ther Med. 2021 Dec;22(6):1470. doi: 10.3892/etm.2021.10905. Epub 2021 Oct 21.
4
Soluble biological markers in osteoarthritis.骨关节炎中的可溶性生物标志物。
Ther Adv Musculoskelet Dis. 2021 Sep 29;13:1759720X211040300. doi: 10.1177/1759720X211040300. eCollection 2021.
5
Transthyretin: From Structural Stability to Osteoarticular and Cardiovascular Diseases.甲状腺素运载蛋白：从结构稳定性到骨关节炎和心血管疾病。
Cells. 2021 Jul 13;10(7):1768. doi: 10.3390/cells10071768.
6
Serum Peptidomic Profile as a Novel Biomarker for Rheumatoid Arthritis.血清肽组学图谱作为类风湿关节炎的一种新型生物标志物
Int J Rheumatol. 2020 Aug 3;2020:6069484. doi: 10.1155/2020/6069484. eCollection 2020.
7
Early urine proteome changes in an implanted bone cancer rat model.植入性骨癌大鼠模型早期尿液蛋白质组变化
Bone Rep. 2019 Dec 13;12:100238. doi: 10.1016/j.bonr.2019.100238. eCollection 2020 Jun.
8
Synovial fluid proteome changes in ACL injury-induced posttraumatic osteoarthritis: Proteomics analysis of porcine knee synovial fluid.前交叉韧带损伤后创伤性骨关节炎关节滑液蛋白质组变化：猪膝关节滑液的蛋白质组学分析。
PLoS One. 2019 Mar 1;14(3):e0212662. doi: 10.1371/journal.pone.0212662. eCollection 2019.
9
Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids.免疫亲和色谱法对人类生物流体深度蛋白质组分析的贡献。
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:57-68. doi: 10.1016/j.jchromb.2016.01.015. Epub 2016 Jan 12.
10
Applying computation biology and "big data" to develop multiplex diagnostics for complex chronic diseases such as osteoarthritis.应用计算生物学和“大数据”来开发针对骨关节炎等复杂慢性疾病的多重诊断方法。
Biomarkers. 2015;20(8):533-9. doi: 10.3109/1354750X.2015.1105499. Epub 2016 Jan 26.
Impaired expression of genes regulating cholesterol efflux in human osteoarthritic chondrocytes.人骨性关节炎软骨细胞中胆固醇外流调节基因表达受损。
J Orthop Res. 2010 Aug;28(8):1033-9. doi: 10.1002/jor.21084.
4
Proteomics role in the search for improved diagnosis, prognosis and treatment of osteoarthritis.蛋白质组学在寻找改善骨关节炎的诊断、预后和治疗中的作用。
Osteoarthritis Cartilage. 2010 Apr;18(4):500-9. doi: 10.1016/j.joca.2009.11.012. Epub 2009 Dec 23.
5
Challenges for biomarker discovery in body fluids using SELDI-TOF-MS.使用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）在体液中发现生物标志物面临的挑战。
J Biomed Biotechnol. 2010;2010:906082. doi: 10.1155/2010/906082. Epub 2009 Dec 6.
6
Insurer and out-of-pocket costs of osteoarthritis in the US: evidence from national survey data.美国骨关节炎的保险费用及自付费用：来自全国调查数据的证据
Arthritis Rheum. 2009 Dec;60(12):3546-53. doi: 10.1002/art.24984.
7
Knee osteoarthritis in obese women with cardiometabolic clustering.患有心脏代谢聚集症的肥胖女性的膝骨关节炎
Arthritis Rheum. 2009 Oct 15;61(10):1328-36. doi: 10.1002/art.24739.
8
Lifetime risk of symptomatic knee osteoarthritis.有症状的膝关节骨关节炎的终生风险。
Arthritis Rheum. 2008 Sep 15;59(9):1207-13. doi: 10.1002/art.24021.
9
Sputum proteomics in inflammatory and suppurative respiratory diseases.炎症性和化脓性呼吸道疾病中的痰液蛋白质组学
Am J Respir Crit Care Med. 2008 Sep 1;178(5):444-52. doi: 10.1164/rccm.200703-409OC. Epub 2008 Jun 19.
10
Changes in surgical loads and economic burden of hip and knee replacements in the US: 1997-2004.1997 - 2004年美国髋关节和膝关节置换手术量及经济负担的变化
Arthritis Rheum. 2008 Apr 15;59(4):481-8. doi: 10.1002/art.23525.