Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie, Dongzhimennei, Beijing 100700, China.
Evid Based Complement Alternat Med. 2013;2013:535742. doi: 10.1155/2013/535742. Epub 2013 Jul 1.
The aim of this study was to explore the "intensity-response" relationship between EAS and the effect of gastric motility of rats and its underlying peripheral neural mechanism by employing ASIC3 knockout (ASIC3-/-), TRPV1 knockout (TRPV1-/-), and C57BL/6 mice. For adult male Sprague-Dawley (n = 18) rats, the intensities of EAS were 0.5, 1, 3, 5, 7, and 9 mA, respectively. For mice (n = 8 in each group), only 1 mA was used, by which C fiber of the mice can be activated. Gastric antrum motility was measured by intrapyloric balloon. Gastric motility was facilitated by EAS at ST36 and inhibited by EAS at CV12. The half maximal facilitation intensity of EAS at ST36 was 2.1-2.3 mA, and the half maximal inhibitory intensity of EAS at CV12 was 2.8 mA. In comparison with C57BL/6 mice, the facilitatory effect of ST36 and inhibitive effect of CV12 in ASIC3-/- mice decreased, but the difference was not statistically significant (P > 0.05). However, these effects in TRPV1-/- mice decreased significantly (P < 0.001). The results indicated that there existed an "intensity-response" relationship between EAS and the effect of gastric motility. TRPV1 receptor was involved in the regulation of gastric motility of EAS.
本研究旨在通过使用 ASIC3 敲除（ASIC3-/-）、TRPV1 敲除（TRPV1-/-）和 C57BL/6 小鼠，探讨 EAS 与大鼠胃动力效应之间的“强度-反应”关系及其潜在的外周神经机制。对于成年雄性 Sprague-Dawley（n = 18）大鼠，EAS 的强度分别为 0.5、1、3、5、7 和 9 mA。对于小鼠（每组 n = 8），仅使用 1 mA，可激活小鼠的 C 纤维。胃窦蠕动通过幽门内球囊测量。EAS 在 ST36 促进胃动力，而在 CV12 抑制胃动力。EAS 在 ST36 的半最大促进强度为 2.1-2.3 mA，EAS 在 CV12 的半最大抑制强度为 2.8 mA。与 C57BL/6 小鼠相比，ASIC3-/- 小鼠 ST36 的促动作用和 CV12 的抑制作用减弱，但差异无统计学意义（P > 0.05）。然而，TRPV1-/- 小鼠的这些作用显著减弱（P < 0.001）。结果表明，EAS 与胃动力效应之间存在“强度-反应”关系。TRPV1 受体参与了 EAS 对胃动力的调节。
