Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Evid Based Complement Alternat Med. 2013;2013:690190. doi: 10.1155/2013/690190. Epub 2013 Jun 27.
Reperfusion therapy is widely utilized for acute myocardial infarction (AMI), but further injury induced by rapidly initiating reperfusion of the heart is often encountered in clinical practice. Ginsenoside RK3 (RK3) is reportedly present in the processed Radix notoginseng that is often used as a major ingredient of the compound preparation for ischemic heart diseases. This study aimed to investigate the possible protective effect of RK3 against hypoxia-reoxygenation (H/R) induced H9c2 cardiomyocytes damage and its underlying mechanisms. Our results showed that RK3 pretreatment caused increased cell viability and decreased levels of LDH leakage compared with the H/R group. Moreover, RK3 pretreatment inhibited cell apoptosis, as evidenced by decreased caspase-3 activity, TUNEL-positive cells, and Bax expression, as well as increased Bcl-2 level. Further mechanism investigation revealed that RK3 prevented H9c2 cardiomyocytes injury and apoptosis induced by H/R via AKT/Nrf-2/HO-1 and MAPK pathways. These observations indicate that RK3 has the potential to exert cardioprotective effects against H/R injury, which might be of great importance to clinical efficacy for AMI treatment.
再灌注疗法广泛应用于急性心肌梗死（AMI），但在临床实践中，心脏迅速再灌注往往会引起进一步的损伤。据报道，人参皂苷 RK3（RK3）存在于常用作缺血性心脏病复方制剂主要成分的炮制三七中。本研究旨在探讨 RK3 对缺氧/复氧（H/R）诱导的 H9c2 心肌细胞损伤的可能保护作用及其潜在机制。研究结果表明，与 H/R 组相比，RK3 预处理可增加细胞活力并降低 LDH 漏出水平。此外，RK3 预处理抑制细胞凋亡，表现为 caspase-3 活性、TUNEL 阳性细胞和 Bax 表达降低，以及 Bcl-2 水平升高。进一步的机制研究表明，RK3 通过 AKT/Nrf-2/HO-1 和 MAPK 通路防止 H/R 诱导的 H9c2 心肌细胞损伤和凋亡。这些观察结果表明，RK3 具有对抗 H/R 损伤的心脏保护作用，这对于 AMI 治疗的临床疗效可能非常重要。
