Universidade Federal do Maranhão (UFMA), 65080-805 São Luís, MA, Brazil ; Rede Nordeste de Biotecnologia (RENORBIO), 65080-805 São Luís, MA, Brazil ; Faculdade Leão Sampaio (FALS), 63180-000 Juazeiro do Norte, CE, Brazil ; Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz (FIOCRUZ), 21040-900 Rio de Janeiro, RJ, Brazil.
Evid Based Complement Alternat Med. 2013;2013:727042. doi: 10.1155/2013/727042. Epub 2013 Jul 9.
The search for new immunopharmacological chemical agents to treat various diseases caused by bacteria, fungi, and protozoa, such as leishmaniasis, for example, has led to the exploration of potential products from plant species and their main active ingredients. Antimonial drugs are the current treatment for leishmaniasis. These drugs cause major side effects and frequent discontinuation of treatment. In this study, we evaluated the in vitro leishmanicidal activity of essential oil of Vanillosmopsis arborea (VAEO) and its major compound α -bisabolol against Leishmania amazonensis. The essential oil and α -bisabolol showed activity against promastigotes (IC50 7.35 and 4.95 μ g/mL resp.) and intracellular amastigotes (IC50 12.58 and 10.70 μ g/mL, resp.). Neither product showed any cytotoxicity on treated macrophages. The ultrastructural analysis of promastigotes incubated with VAEO or α -bisabolol at 30 μ g/mL, showed morphological changes with the accumulation of vesicles electrodense lipid inclusions. The results give evidence that both VAEO and α -bisabolol have potential as new therapeutic agents against leishmaniasis.
为了寻找治疗由细菌、真菌和原生动物引起的各种疾病（如利什曼病）的新免疫药理学化学药物，人们开始探索植物物种及其主要活性成分的潜在产品。锑剂是目前治疗利什曼病的方法。这些药物会引起严重的副作用，导致治疗频繁中断。在这项研究中，我们评估了 Vanillosmopsis arborea（VAEO）精油及其主要化合物 α - 倍半水芹醇对 Leishmania amazonensis 的体外杀利什曼原虫活性。精油和 α - 倍半水芹醇对前鞭毛体（IC50 分别为 7.35 和 4.95μg/mL）和内阿米巴体（IC50 分别为 12.58 和 10.70μg/mL）均具有活性。两种产品在处理后的巨噬细胞上均无细胞毒性。在 30μg/mL 浓度下，用 VAEO 或 α - 倍半水芹醇孵育的前鞭毛体的超微结构分析显示，形态发生变化，电致密脂囊泡积累。这些结果表明，VAEO 和 α - 倍半水芹醇均有可能成为治疗利什曼病的新疗法。
