Seguin Johanne, Doan Bich-Thuy, Latorre Ossa Heldmuth, Jugé Lauriane, Gennisson Jean-Luc, Tanter Mickaël, Scherman Daniel, Chabot Guy G, Mignet Nathalie
Chemical, Genetic and Imaging Pharmacology Laboratory, Faculty of Pharmacy, Chimie ParisTech, Paris Descartes University, Sorbonne Paris Cité, INSERM U1022, CNRS UMR8151, 4 Avenue de l'Observatoire, 75006 Paris, France.
Int J Mol Imaging. 2013;2013:983534. doi: 10.1155/2013/983534. Epub 2013 Jul 2.
Background and Objectives. To determine the most appropriate technique for tumour followup in experimental therapeutics, we compared ultrasound (US) and magnetic resonance imaging (MRI) to characterize ectopic and orthotopic colon carcinoma models. Methods. CT26 tumours were implanted subcutaneously (s.c.) in Balb/c mice for the ectopic model or into the caecum for the orthotopic model. Tumours were evaluated by histology, spectrofluorescence, MRI, and US. Results. Histology of CT26 tumour showed homogeneously dispersed cancer cells and blood vessels. The visualization of the vascular network using labelled albumin showed that CT26 tumours were highly vascularized and disorganized. MRI allowed high-resolution and accurate 3D tumour measurements and provided additional anatomical and functional information. Noninvasive US imaging allowed good delineation of tumours despite an hypoechogenic signal. Monitoring of tumour growth with US could be accomplished as early as 5 days after implantation with a shorter acquisition time (<5 min) compared to MRI. Conclusion. MRI and US afforded excellent noninvasive imaging techniques to accurately follow tumour growth of ectopic and orthotopic CT26 tumours. These two techniques can be appropriately used for tumour treatment followup, with a preference for US imaging, due to its short acquisition time and simplicity of use.
背景与目的。为确定实验性治疗中肿瘤随访的最合适技术，我们比较了超声（US）和磁共振成像（MRI）以表征异位和原位结肠癌模型。方法。将CT26肿瘤皮下植入（s.c.）Balb/c小鼠用于异位模型，或植入盲肠用于原位模型。通过组织学、光谱荧光、MRI和US对肿瘤进行评估。结果。CT26肿瘤的组织学显示癌细胞和血管均匀分散。使用标记白蛋白对血管网络的可视化显示CT26肿瘤血管高度丰富且结构紊乱。MRI能够进行高分辨率和准确的三维肿瘤测量，并提供额外的解剖和功能信息。尽管信号为低回声，但无创US成像仍能很好地勾勒出肿瘤轮廓。与MRI相比，早在植入后5天即可通过US完成肿瘤生长监测，采集时间更短（<5分钟）。结论。MRI和US提供了出色的无创成像技术，可准确跟踪异位和原位CT26肿瘤的生长。这两种技术可适用于肿瘤治疗随访，由于US成像采集时间短且使用简便，因此更倾向于使用US成像。
