Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Center for Systems Biology Dresden, Dresden, Germany.
PLoS Comput Biol. 2018 Apr 13;14(4):e1006079. doi: 10.1371/journal.pcbi.1006079. eCollection 2018 Apr.
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.
在光片显微镜中，样品诱导的图像退化仍然是一个复杂的波动光学问题。在这里，我们介绍了 biobeam，这是一个开源软件包，通过结合来自 105-106 个复用和 GPU 加速的点扩散函数计算的数据，它可以模拟运行中的光片显微镜。这些模拟的波动光学性质导致对空间变化的像差、衍射伪影、几何图像失真、自适应光学和新兴的波动光学现象的忠实再现，并使光片显微镜中的图像形成在计算上变得可行。
