体外研究磷霉素对来自 16 家西班牙医院的临床铜绿假单胞菌分离株的活性：确证标准肉汤微量稀释法作为药敏试验方法的有效性。
In vitro activity of fosfomycin against a collection of clinical Pseudomonas aeruginosa isolates from 16 Spanish hospitals: establishing the validity of standard broth microdilution as susceptibility testing method.
机构信息
Servicio de Microbiología, Hospital Universitario Ramón y Cajal, and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
出版信息
Antimicrob Agents Chemother. 2013 Nov;57(11):5701-3. doi: 10.1128/AAC.00589-13. Epub 2013 Aug 12.
Abstract
The broth microdilution method for fosfomycin and Pseudomonas aeruginosa was assessed and compared with the approved agar dilution method in 206 genetically unrelated P. aeruginosa clinical isolates. Essential agreement between the two methods was 84%, and categorical agreement was 89.3%. Additionally, Etest and disk diffusion assays were performed. Results validate broth microdilution as a reliable susceptibility testing method for fosfomycin against P. aeruginosa. Conversely, unacceptable concordance was established between Etest and disk diffusion results with agar dilution results.
摘要
采用肉汤微量稀释法检测 206 株非重复的铜绿假单胞菌临床分离株对磷霉素的敏感性，并与琼脂稀释法进行比较。两种方法的符合率为 84%，一致性为 89.3%。此外，还进行了 Etest 和纸片扩散法检测。结果表明，肉汤微量稀释法是一种可靠的药敏检测方法，适用于检测磷霉素对铜绿假单胞菌的敏感性。相反，Etest 和纸片扩散法与琼脂稀释法的结果之间存在不可接受的一致性。
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