CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
PLoS One. 2013 Aug 5;8(8):e70573. doi: 10.1371/journal.pone.0070573. Print 2013.
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.
诱导多能干细胞（iPS 细胞）在再生医学、药物发现和疾病建模等领域具有巨大的应用潜力。我们在这里描述了一种在无饲养层、无病毒、无血清条件下，不使用癌基因 c-MYC，从尿液来源的细胞（UCs）中生成人 iPS 细胞的实用方法。我们表明，这种方法可以应用于具有不同遗传背景的大量人群。UCs 易于获取，并且具有高重编程效率，相对于其他用于生成 iPS 细胞的细胞类型具有优势。使用本研究中描述的方法，我们从具有不同遗传背景的 20 个供体中生成了 93 个 iPS 细胞系。该非病毒 iPS 细胞库为 iPS 细胞研究提供了宝贵的资源，促进了人类 iPS 细胞的未来应用。
