Shelton Pernille Tofteng, Jensen Knud J
IGM, Faculty of Life Sciences, University of Copenhagen, Zealand Pharma, Glostrup, Denmark.
Methods Mol Biol. 2013;1047:23-41. doi: 10.1007/978-1-62703-544-6_2.
This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N (α)-protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C-terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.
本章介绍了以芴甲氧羰基（Fmoc）基团作为N（α）-保护基的固相肽合成基本方案（Fmoc-SPPS）。本章介绍了树脂及其处理方法、连接子的选择以及肽链组装的最常用方法。为固相合成正确选择树脂和连接子是成功进行肽合成的关键参数。本章概述了用于合成具有C端酰胺、羧酸等的肽的最常见和有用的树脂及连接子。本章最后给出了通用固相肽合成的可靠方案，即标准操作。
