Centro de Investigaciones Biológicas, CSIC, c/Ramiro de Maeztu 9, 28040, Madrid, Spain.
Environ Microbiol. 2013 Dec;15(12):3158-68. doi: 10.1111/1462-2920.12214. Epub 2013 Aug 15.
The use of artificial lipid membranes, structured as giant unilamellar vesicles (GUVs), provides the opportunity to investigate membrane-associated biological processes under defined experimental conditions. Due to their large size, they are uniquely adapted to investigate the properties and organization (in time and space) of macromolecular complexes incorporated in the vesicle interior by imaging and micro-spectroscopic techniques. Experimental methods to produce giant vesicles and to encapsulate proteins inside them are here reviewed. Previous experimental work to reconstitute elements of the bacterial division machinery in these membrane-like systems is summarized. Future challenges towards reconstructing minimal divisome assemblies in giant vesicles as cytomimetic containers are discussed.
使用人工脂质膜，构建成巨大的单层囊泡（GUVs），为在特定实验条件下研究膜相关的生物过程提供了机会。由于其较大的尺寸，它们特别适合通过成像和微光谱技术研究包含在囊泡内部的大分子复合物的性质和组织（在时间和空间上）。这里综述了制备巨大囊泡和将蛋白质包裹在其内的实验方法。总结了以前在这些类似膜的系统中重建细菌分裂机制元件的实验工作。讨论了在巨大囊泡中作为细胞模拟容器重建最小分裂体组装的未来挑战。
