Zhou Shu-Yuan, Wang Ying-Han, Li Yu-Jie, Yang Qing, Gong Zi-Peng, Ruan Cong-Xiao, Kan Xiao-Xi, Zhang Rui-Jie, Zhu Xiao-Xin
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1595-600.
Lipid accumulation in the vessel wall and tunica intima vasorum pathological changes are important factors in the development of atherosclerosis, which are closely related with hemodynamics. In this paper, we established a model of local low shear stress in rabbits using carotid artery cannula and a high cholesterol diet for 2 weeks, 4 weeks and 8 weeks. The effects of Shenlian extract on blood flow, vascular pathology formation and lipid metabolism were assessed by electromagnetic blood flow meter and hematoxylin-eosin staining of the proximal end in carotid artery at different times. The results demonstrate that the relationship between blood flow and shear stress for control, atorvastatin, Shenlian extract high-dose, Shenlian extract middle-dose, and Shenlian extract low-dose were linearly related. The blood flow and the shear stress of proximal end in carotid artery of Shenlian extract (1.12, 2.24, 4.48 g x kg(-1)), and atorvastatin (4.7 x 10(-4) g x kg(-1)) were significantly (P < 0.05)increased compared with the control. Plasma total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) ,and high density lipoprotein cholesterol (HDL-C) were markedly decreased with the increasing of dose and time. This study is the first to prove that the inhibition of Shenlian extract on low shear stress (LSS) induces rabbits carotid atherosclerosis with increasing blood flow and decreasing lipids and vessel pathological changes.
血管壁脂质蓄积和血管内膜病理变化是动脉粥样硬化发展的重要因素，与血流动力学密切相关。在本文中，我们使用颈动脉插管和高胆固醇饮食对兔子进行2周、4周和8周的处理，建立局部低切应力模型。通过电磁血流仪以及对不同时间点颈动脉近端进行苏木精-伊红染色，评估参莲提取物对血流、血管病理形成和脂质代谢的影响。结果表明，对照组、阿托伐他汀、参莲提取物高剂量组、参莲提取物中剂量组和参莲提取物低剂量组的血流与切应力之间呈线性关系。参莲提取物（1.12、2.24、4.48 g·kg⁻¹）和阿托伐他汀（4.7×10⁻⁴ g·kg⁻¹）处理的兔子颈动脉近端的血流和切应力与对照组相比显著增加（P < 0.05）。随着剂量和时间的增加，血浆总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）显著降低。本研究首次证明参莲提取物对低切应力（LSS）的抑制作用可通过增加血流、降低血脂和血管病理变化来诱导兔子颈动脉粥样硬化。
