Affliated Hospital of Zunyi Medical College, Department of Thoracic and Cardiovascular Surgery, Zunyi, Guizhou 563003, China.
World J Surg Oncol. 2013 Aug 16;11:199. doi: 10.1186/1477-7819-11-199.
Nuclear factor (erythroid-derived 2)-like (Nrf)2 and metallothionein have been implicated in carcinogenesis. This study investigated the expression of Nrf2 and of Nrf2-targeted genes (NQO1 and GCLC) and the genes for the metallothionein (MT) isoforms (MT-1A and MT-2A) in human lung cancer and cancer-surrounding tissues.
Surgically removed lung cancer samples (n = 80) and cancer-surrounding tissues (n = 38) were collected from Zunyi Medical College Hospital, China. Total RNA was extracted, purified, and used for real-time reverse transcription-PCR analysis of interested genes.
Expression of the Nrf2-targed genes NQO1 and GCLC tended to be higher (30 to 60%) in lung cancers, but was not significantly different from that in peri-cancer tissues. By contrast, expression of the genes for M)-1A, MT-2A, and the metal transcription factor MTF-1 were three-fold to four-fold lower in lung cancers.
In surgical samples of lung cancer, MT expression was generally downregulated, whereas Nrf2 expression tended to be upregulated. These changes could play an integral role in lung carcinogenesis.
核因子（红细胞衍生 2 样）（Nrf）2 和金属硫蛋白已被牵涉到癌症发生中。本研究调查了 Nrf2 及其 Nrf2 靶向基因（NQO1 和 GCLC）以及金属硫蛋白（MT）同工型（MT-1A 和 MT-2A）的基因在人肺癌和癌旁组织中的表达。
从中国遵义医学院附属医院采集了 80 例肺癌手术切除样本和 38 例癌旁组织。提取、纯化总 RNA，用于实时逆转录聚合酶链反应分析感兴趣的基因。
Nrf2 靶向基因 NQO1 和 GCLC 的表达在肺癌中趋于升高（30%至 60%），但与癌旁组织无显著差异。相比之下，M）-1A、MT-2A 和金属转录因子 MTF-1 的基因表达在肺癌中低了三到四倍。
在肺癌的手术样本中，MT 的表达通常下调，而 Nrf2 的表达趋于上调。这些变化可能在肺癌发生中起重要作用。
