Department of Preventive Medicine and Medical Research Institute, College of Medicine, Chungbuk National University, 52 Naesudong-ro, Heungdok-gu, Cheongju, 361-763, Korea.
Cancer Causes Control. 2013 Nov;24(11):1963-72. doi: 10.1007/s10552-013-0272-3. Epub 2013 Aug 15.
We investigated the effects of aflatoxin B1 (AFB1) intake, genetic polymorphisms of AFB1 metabolic enzymes, and interactions between the polymorphisms and intake of AFB1 with regard to the risk of gastric cancer in Korean.
The participants in the study included 477 gastric cancer patients and 477 age- and sex-matched control subjects. Direct interviews and a structured questionnaire were used to determine the level of exposure to AFB1, and the GoldenGate assay and multiplex polymerase chain reaction were used for genotypic analyses of the cytochrome P450 1A2 (CYP1A2), cytochrome P450 1E1, epoxide hydrolase 1, and glutathione S-transferase genes.
The probable daily intake of AFB1 was significantly higher among gastric cancer patients than among control subjects (cases vs. controls: 1.91 ± 0.87 vs. 1.65 ± 0.72 ng/kg bw/day, p < 0.0001), and increased AFB1 intake was significantly associated with an elevated risk of gastric cancer (odds ratio 1.94; 95 % confidence interval 1.43-2.63). However, genetic polymorphisms of AFB1 metabolic enzymes were not associated with gastric cancer, with the exception of CYP1A2. Moreover, there was no interaction between AFB1 intake and the genotypes of metabolic enzymes that affect gastric cancer risk.
Our results suggest that dietary AFB1 exposure might be associated with a risk of gastric cancer. However, the effect of AFB1 on gastric carcinogenesis may not be modulated by genetic polymorphisms of AFB1 metabolic enzymes.
我们研究了黄曲霉毒素 B1（AFB1）摄入、AFB1 代谢酶的遗传多态性以及多态性与 AFB1 摄入之间的相互作用对韩国人胃癌风险的影响。
本研究的参与者包括 477 名胃癌患者和 477 名年龄和性别匹配的对照者。直接访谈和结构化问卷用于确定 AFB1 的暴露水平，金盖尔检测和多重聚合酶链反应用于细胞色素 P450 1A2（CYP1A2）、细胞色素 P450 1E1、环氧化物水解酶 1 和谷胱甘肽 S-转移酶基因的基因型分析。
胃癌患者的 AFB1 可能每日摄入量明显高于对照者（病例 vs. 对照：1.91 ± 0.87 vs. 1.65 ± 0.72 ng/kg bw/day，p < 0.0001），并且 AFB1 摄入增加与胃癌风险升高显著相关（比值比 1.94；95%置信区间 1.43-2.63）。然而，AFB1 代谢酶的遗传多态性与胃癌无关，除了 CYP1A2 之外。此外，AFB1 摄入与影响胃癌风险的代谢酶的基因型之间没有相互作用。
我们的结果表明，饮食中 AFB1 的暴露可能与胃癌风险相关。然而，AFB1 对胃癌发生的影响可能不受 AFB1 代谢酶遗传多态性的调节。
