Eco-Innovation Research Centre, Department of Chemical & Life Sciences, Waterford Institute of Technology, Cork Road, Waterford, Ireland.
Int J Pharm. 2013 Nov 1;456(1):10-20. doi: 10.1016/j.ijpharm.2013.08.018. Epub 2013 Aug 16.
The objective of this study was to develop a novel antimicrobial seaweed wound dressing. The seaweed extract was active against nine clinically-relevant wound pathogens. A hydrogel formulation was prepared using polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), followed by addition of 1% seaweed extract. The antimicrobial properties of the novel dressing were tested using agar diffusion assays, with release-profiles examined using gel leaching and gel transfer assays. The dressing was found to be effective against the same microbial strains as the seaweed extract, with similar efficacy to the commonly used silver-based dressing, Acticoat(®). Antimicrobial release-profile assays revealed that the dressing was effective in inhibiting 70-90% of the bacterial population within the first 30 min, followed by a long, sustained released up to 97 h, without leaving a residue following five subsequent transfers of the dressing. Antimicrobial activity was stable for up to 6 months of storage at 4 °C, but activity was reduced slightly after 15 weeks. Following autoclave sterilization, the dressing displayed a slower release profile compared to a non-autoclaved counterpart. Hence, the seaweed dressing may have commercial applications, potentially competing with silver-based dressings at a lower cost per-application. This is the first report of development of a seaweed-based antimicrobial dressing.
本研究旨在开发一种新型抗菌海藻创伤敷料。该海藻提取物对 9 种临床相关的伤口病原体具有活性。使用聚乙烯醇（PVA）和聚乙烯吡咯烷酮（PVP）制备水凝胶制剂，然后加入 1%的海藻提取物。使用琼脂扩散试验测试新型敷料的抗菌性能，并使用凝胶浸出和凝胶转移试验检查释放曲线。研究发现，这种敷料对与海藻提取物相同的微生物菌株有效，与常用的银基敷料 Acticoat(®)具有相似的功效。抗菌释放曲线试验表明，该敷料在最初 30 分钟内有效抑制 70-90%的细菌种群，然后持续释放长达 97 小时，在随后的五次敷料转移后没有留下残留物。在 4°C 下储存长达 6 个月时，抗菌活性稳定，但在 15 周后略有下降。经过高压蒸汽灭菌后，与未经高压蒸汽灭菌的敷料相比，敷料的释放曲线较慢。因此，这种海藻敷料具有商业应用的潜力，可能以更低的每应用成本与银基敷料竞争。这是首次报道开发基于海藻的抗菌敷料。
