El-Fawal Gomaa F, Yassin Abdelrahman M, El-Deeb Nehal M
Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technology Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
Biopharmaceutical Product Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
AAPS PharmSciTech. 2017 Jul;18(5):1605-1616. doi: 10.1208/s12249-016-0628-6. Epub 2016 Sep 12.
Material barrier properties to microbes are an important issue in many pharmaceutical applications like wound dressings. A wide range of biomaterials has been used to manage the chronic inflamed wounds. Eight hydrogel membranes of poly vinyl alcohol (PVA) with κ-carrageenan (KC) and Lactobacillus bulgaricus extract (LAB) have been prepared by using freeze-thawing technique. To evaluate the membranes efficiency as wound dressing agents, various tests have been done like gel fraction, swelling behavior, mechanical properties, etc. The antibacterial activities of the prepared membranes were tested against the antibiotic-resistant bacterial isolates. In addition, the safety usage of the prepared hydrogel was checked on human dermal fibroblast cells. The anti-inflammatory properties of the prepared hydrogel on LPS-PBMC cell inflammatory model were quantified using enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-qPCR). The analysis data of TGA, SEM, gel fraction, and swelling behavior showed changes in properties of prepared PVA\KC\LAB hydrogel membrane than pure PVA hydrogel membrane. The antibacterial activities of the prepared membranes augmented in LAB extract-prepared membranes. Out of the eight used hydrogel membranes, the PVAKC4 hydrogel membrane is the safest one on fibroblast cellular proliferation with a maximum proliferation percentage 97.3%. Also, all the used hydrogel membrane showed abilities to reduce the concentration of IL-2 and IL-8 compared with both negative and positive control. In addition, almost all the prepared hydrogel membrane showed variable abilities to downregulate the expression of TNF-α gene with superior effect of hydrogel membrane KC1. PVA/KC/LAB extract hydrogel membrane may be a promising material for wound dressing application and could accelerate the healing process of the chronic wound because of its antimicrobial and anti-inflammatory properties.
对微生物的材料阻隔性能在许多药物应用（如伤口敷料）中是一个重要问题。多种生物材料已被用于处理慢性炎症伤口。通过冻融技术制备了八种含有κ-卡拉胶（KC）和保加利亚乳杆菌提取物（LAB）的聚乙烯醇（PVA）水凝胶膜。为了评估这些膜作为伤口敷料剂的效率，进行了各种测试，如凝胶分数、溶胀行为、机械性能等。测试了所制备膜对耐抗生素细菌分离株的抗菌活性。此外，还在人皮肤成纤维细胞上检查了所制备水凝胶的安全使用情况。使用酶联免疫吸附测定（ELISA）和实时聚合酶链反应（RT-qPCR）对所制备水凝胶在LPS-PBMC细胞炎症模型上的抗炎特性进行了定量。热重分析（TGA）、扫描电子显微镜（SEM）、凝胶分数和溶胀行为的分析数据表明，与纯PVA水凝胶膜相比，所制备的PVA\KC\LAB水凝胶膜的性能发生了变化。所制备膜的抗菌活性在含有LAB提取物的膜中有所增强。在使用的八种水凝胶膜中，PVAKC4水凝胶膜对成纤维细胞增殖最安全，最大增殖百分比为97.3%。此外，与阴性和阳性对照相比，所有使用的水凝胶膜都显示出降低IL-2和IL-8浓度的能力。此外，几乎所有制备的水凝胶膜都显示出下调TNF-α基因表达的不同能力，其中水凝胶膜KC1的效果最佳。PVA/KC/LAB提取物水凝胶膜因其抗菌和抗炎特性，可能是一种有前途的伤口敷料应用材料，并可加速慢性伤口的愈合过程。
