Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Comp Biochem Physiol B Biochem Mol Biol. 2013 Oct;166(2):133-40. doi: 10.1016/j.cbpb.2013.07.009. Epub 2013 Aug 17.
Three leptin genes (jlLEP-A1, jlLEP-A2 and jlLEP-B) were cloned and characterized in Jian carp (Cyprinus carpio var. Jian), with a highly conserved organization encoded by two exons and one intron. jlLEP-A1 and jlLEP-A2 contained 93 and 102bp introns, respectively, encoding a 171-amino acid protein. jlLEP-B contained a 687bp intron encoding a 168-aa protein. jlLEP-A1 and jlLEP-A2 shared 82.5% identity, but only 29.2% and 28.6% with jlLEP-B, respectively. jlLEP-A1, jlLEP-A2, and jlLEP-B were expressed in a wide range of tissues, but the expression pattern differed between juveniles and adults (and females and males). In juveniles, the expression of jlLEP-A1 and jlLEP-B was higher than jlLEP-A2, and was the highest in the liver and gonad. In females, jlLEP-A1 and jlLEP-A2 expression was the highest in the hypothalamus and liver, whereas jlLEP-B mRNA was detected at low levels in all tissues. In males, jlLEP-A1 mRNA was expressed primarily in the hypothalamus, with only very low levels in the peripheral tissues. jlLEP-A2 and jlLEP-B mRNA were primarily expressed in the muscle, hypothalamus, and liver. The expression of jlLEP-A1 and jlLEP-B mRNA was high in the ovary and testis, respectively. Our results suggest that leptins play an important physiological role in reproduction.
三个瘦素基因（jlLEP-A1、jlLEP-A2 和 jlLEP-B）在剑鱼（鲤鱼 var. Jian）中被克隆和表征，具有由两个外显子和一个内含子编码的高度保守组织。jlLEP-A1 和 jlLEP-A2 分别含有 93bp 和 102bp 内含子，编码 171 个氨基酸的蛋白质。jlLEP-B 含有 687bp 内含子，编码 168 个氨基酸的蛋白质。jlLEP-A1 和 jlLEP-A2 具有 82.5%的同一性，但与 jlLEP-B 分别只有 29.2%和 28.6%的同一性。jlLEP-A1、jlLEP-A2 和 jlLEP-B 在广泛的组织中表达，但在幼鱼和成鱼（以及雌性和雄性）之间的表达模式不同。在幼鱼中，jlLEP-A1 和 jlLEP-B 的表达高于 jlLEP-A2，在肝脏和性腺中最高。在雌性中，jlLEP-A1 和 jlLEP-A2 的表达在下丘脑和肝脏中最高，而 jlLEP-B mRNA 在所有组织中均检测到低水平。在雄性中，jlLEP-A1 mRNA 主要在下丘脑表达，在外周组织中仅表达极低水平。jlLEP-A2 和 jlLEP-B mRNA 主要在肌肉、下丘脑和肝脏中表达。jlLEP-A1 和 jlLEP-B mRNA 的表达在卵巢和睾丸中均较高。我们的结果表明，瘦素在生殖中发挥重要的生理作用。
