Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning 530021, China.
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning 530021, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2019 Jun;30:71-80. doi: 10.1016/j.cbd.2019.01.013. Epub 2019 Jan 30.
The rice flower carp (Cyprinus carpio var. Quanzhounensis) is a bony fish (superclass Osteichthyes), with very soft bones that is a significant feature unlike the other carp species. In this study, we analyzed the mRNA and microRNA (miRNA) transcriptomes in the intermuscular bones of rice flower carp and Jian carp (Cyprinus carpio var. Jian) (a typical member of common carp in China), using Illumina RNA sequencing. We identified 55,340 genes (including 47,541 known genes and 8231 predicted new genes) and 662 miRNAs (including 595 known miRNAs and 67 novel miRNAs) in the two species. By comparing the transcriptomes of the two species, we identified 1523 differentially expressed genes (DEGs) (including 576 up - and 947 downregulated DEGs) and 352 differentially expressed miRNAs (DEMs) (including 85 up- and 267 downregulated DEMs). According to the Gene Ontology (GO) annotation, 7 DEGs and 12 DEMs were found to be involved in the regulation of bone mineralization. The results of this study improve our understanding of the mRNA and miRNA profiles of carp bones, particularly those of the rice flower carp.
泉洲花鯽（Cyprinus carpio var. Quanzhounensis）是一种硬骨鱼类（超纲 Osteichthyes），其骨骼非常柔软，这是与其他鲤鱼品种的显著特征。在这项研究中，我们使用 Illumina RNA 测序分析了泉洲花鯽和建鯽（Cyprinus carpio var. Jian）（中国典型的鲤鱼品种）的肌肉间骨中的 mRNA 和 microRNA（miRNA）转录组。我们在这两个物种中鉴定出了 55340 个基因（包括 47541 个已知基因和 8231 个预测新基因）和 662 个 miRNA（包括 595 个已知 miRNA 和 67 个新 miRNA）。通过比较这两个物种的转录组，我们鉴定出了 1523 个差异表达基因（DEGs）（包括 576 个上调和 947 个下调 DEGs）和 352 个差异表达 miRNA（DEM）（包括 85 个上调和 267 个下调 DEM）。根据基因本体论（GO）注释，有 7 个 DEG 和 12 个 DEM 被发现参与了骨矿化的调节。这项研究的结果提高了我们对鲤鱼骨骼中 mRNA 和 miRNA 谱的理解，特别是对泉洲花鯽的理解。
